| Size | Price | Stock | Qty |
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| 1mg |
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| 5mg |
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JAK-IN-1 is a novel and potent JAK1/2/3 inhibitor with IC50s of 0.26, 0.8 and 3.2 nM, respectively. JAK-IN-1 shows improved selectivity for JAK3 over JAK1.
| ln Vitro |
JAK-IN-1 suppresses the growth of human CD4 and CD8 T cells in a dose-dependent manner following stimulation with anti-CD3/anti-CD28 antibody-coated beads, emulating in part the activation signals that antigen-presenting cells give to T cells. T cells, one of the main cell types for which JAK3 may be important, are used in both cell-based mechanistic and functional experiments where JAK-IN-1 is active [1].
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| ln Vivo |
Greater inhibitory potency for JAK1/JAK3-driven signaling in whole blood assays as opposed to JAK2- or JAK1/JAK2/TYK2-driven signaling indicates that JAK-IN-1 is specific for JAK3 in vivo. JAK-IN-1 dose-dependent inhibition of IL-2-stimulated JAK-IN-1 plasma concentrations was observed. In a dosage- and concentration-dependent manner, JAK-IN-1 inhibits IL-2-driven STAT5 phosphorylation; at a dose of 10 mg/kg (plasma concentration ~480 nM), roughly 50% inhibition is seen [1].
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| References |
| Molecular Formula |
C20H24N6O2
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|---|---|
| Molecular Weight |
380.443563461304
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| Exact Mass |
380.196
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| CAS # |
1334673-53-8
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| PubChem CID |
54589404
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| Appearance |
White to off-white solid powder
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| LogP |
1
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| Hydrogen Bond Donor Count |
2
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| Hydrogen Bond Acceptor Count |
5
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| Rotatable Bond Count |
5
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| Heavy Atom Count |
28
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| Complexity |
685
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| Defined Atom Stereocenter Count |
1
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| SMILES |
O=C([C@@H](C(C)(C)C)NC(C1=CNC2=C1N=C(C=N2)C1CC1)=O)N1CC(C#N)C1
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| InChi Key |
SIEMFXMJGAMFQR-INIZCTEOSA-N
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| InChi Code |
InChI=1S/C20H24N6O2/c1-20(2,3)16(19(28)26-9-11(6-21)10-26)25-18(27)13-7-22-17-15(13)24-14(8-23-17)12-4-5-12/h7-8,11-12,16H,4-5,9-10H2,1-3H3,(H,22,23)(H,25,27)/t16-/m0/s1
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| Chemical Name |
N-[(2R)-1-(3-cyanoazetidin-1-yl)-3,3-dimethyl-1-oxobutan-2-yl]-2-cyclopropyl-5H-pyrrolo[2,3-b]pyrazine-7-carboxamide
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ~66.67 mg/mL (~175.24 mM)
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| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 1.43 mg/mL (3.76 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 14.3 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 1.43 mg/mL (3.76 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 14.3 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.6285 mL | 13.1427 mL | 26.2854 mL | |
| 5 mM | 0.5257 mL | 2.6285 mL | 5.2571 mL | |
| 10 mM | 0.2629 mL | 1.3143 mL | 2.6285 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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