| Size | Price | Stock | Qty |
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| 5mg |
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| 10mg |
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| 25mg |
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| 50mg |
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| 100mg |
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| 250mg |
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Purity: ≥98%
JI051 is an inhibitor of the cancer-associated transcription factor Hes1 via the PHB2 chaperone. JI051 stabilizes PHB2's interaction with Hes1 outside the nucleus, inducing G2/M cell cycle arrest.
| ln Vitro |
G2/M cell cycle arrest is caused by JI051 [1]. HEK293 cell growth is markedly inhibited by JI051 (0.1-10 µM, 24 hours) with an EC50 of 0.3 µM [1].
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|---|---|
| Cell Assay |
Cell Proliferation Assay[1]
Cell Types: HEK293 Cell Tested Concentrations: 0.1-10 µM Incubation Duration: 24 hrs (hours) Experimental Results: Dose-dependent inhibition of HEK293 cell proliferation at low concentrations. |
| References |
[1]. Perron A, et al. Small-molecule screening yields a compound that inhibits the cancer-associated transcription factor Hes1 via the PHB2 chaperone. J Biol Chem. 2018 May 25;293(21):8285-8294.
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| Molecular Formula |
C22H24N2O3
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|---|---|
| Molecular Weight |
364.45
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| Exact Mass |
364.1787
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| CAS # |
2234281-75-3
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| Related CAS # |
2234281-75-3;
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| SMILES |
O=C(NCCC1=CC=CC=C1OCC)/C=C/C(N2)=CC3=C2C(OC)=CC=C3
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| InChi Key |
XIXIBPRUNJKZQI-VAWYXSNFSA-N
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| InChi Code |
InChI=1S/C22H24N2O3/c1-3-27-19-9-5-4-7-16(19)13-14-23-21(25)12-11-18-15-17-8-6-10-20(26-2)22(17)24-18/h4-12,15,24H,3,13-14H2,1-2H3,(H,23,25)/b12-11+
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| Chemical Name |
(E)-N-(2-Ethoxyphenethyl)-3-(7-methoxy-1H-indol-2-yl)acrylamide
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| Synonyms |
JI051 JI-051 JI 051
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ~125 mg/mL (~342.99 mM)
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|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.08 mg/mL (5.71 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.08 mg/mL (5.71 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.7439 mL | 13.7193 mL | 27.4386 mL | |
| 5 mM | 0.5488 mL | 2.7439 mL | 5.4877 mL | |
| 10 mM | 0.2744 mL | 1.3719 mL | 2.7439 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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