| Size | Price | Stock | Qty |
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| 50mg |
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| 100mg |
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| Other Sizes |
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| ln Vitro |
Puerarin suppressed the mRNA of these cells in RT-PCR assays using RAW264.7, as well as the LPS-induced expression of iNOS, COX-2 cells, and CRP. I-κB phosphorylation and puerarin-mediated regulation of LPS-induced iNOS, COX-2, and CRP expression drift from the transcriptional level to regulate NF-κB activation are the causes of the inhibition of iNOS, COX-2, and CRP expression [1]. It is a novel kind of IK1 open channel blocker and could be the source of puerarin's antiarrhythmic action. IK1 current is dynamically inhibited by purerarin, an open channel inhibitor of IK1 [2].
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| ln Vivo |
Due to their possible antioxidant, anti-inflammatory, or anti-cellular properties, genistein and puerarin both successfully reduce liver damage brought on by long-term alcohol-activated pathways. Genistein also decreased malondialdehyde (1.05±0.0947 vs. 1.28±0.213 nmol/mg pro, p<0.05), tumor cytokine α (3.12±0.498 vs. 3.82±0.277 pg/mg pro, p < 0.05), and interleukin-6 (1.46±0.223 vs. 1.88±0.309 pg/mg pro), p < 0.05). On the other hand, puerarin was superior to genistein in terms of raising serum activity or propionate aminotransferase levels (35.8±3.95 vs. 42.6) ±6.56 U/L, p < 0.05) and LDL carrot (1.12±0.160 vs. 1.55±0.150 mmol/L, p < 0.05)[3]. Puerarin has been shown to considerably ameliorate early renal damage [4], maybe through blocking the production of TNF-α and ICAM-1 in diabetic renin.
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| References |
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| Additional Infomation |
Puerarin is a hydroxyisoflavone that is isoflavone substituted by hydroxy groups at positions 7 and 4' and a beta-D-glucopyranosyl residue at position 8 via a C-glycosidic linkage. It has a role as a plant metabolite, an autophagy inducer, a cardioprotective agent, an antioxidant, an anti-inflammatory agent, an antipyretic and a ferroptosis inhibitor. It is a C-glycosyl compound and a hydroxyisoflavone. It is functionally related to an isoflavone. It is a conjugate acid of a puerarin(1-).
Puerarin has been investigated for the treatment of Alcohol Abuse. Puerarin has been reported in Bupleurum chinense, Pueraria calycina, and other organisms with data available. |
| Molecular Formula |
C21H20O9
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|---|---|
| Molecular Weight |
416.3781
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| Exact Mass |
416.1107
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| CAS # |
3681-99-0
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| PubChem CID |
5281807
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| Appearance |
White to off-white solid powder
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| Density |
1.6±0.1 g/cm3
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| Boiling Point |
791.2±60.0 °C at 760 mmHg
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| Melting Point |
187-189°C
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| Flash Point |
281.5±26.4 °C
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| Vapour Pressure |
0.0±2.9 mmHg at 25°C
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| Index of Refraction |
1.717
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| LogP |
-0.67
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| Hydrogen Bond Donor Count |
6
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| Hydrogen Bond Acceptor Count |
9
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| Rotatable Bond Count |
3
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| Heavy Atom Count |
30
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| Complexity |
659
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| Defined Atom Stereocenter Count |
5
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| SMILES |
C1=CC(=CC=C1C2=COC3=C(C2=O)C=CC(=C3[C@H]4[C@@H]([C@H]([C@@H]([C@H](O4)CO)O)O)O)O)O
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| Synonyms |
NPI 031G; NPI031G; NPI-031G
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ~50 mg/mL (~120.08 mM)
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|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 3 mg/mL (7.20 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 30.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 3 mg/mL (7.20 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 30.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. View More
Solubility in Formulation 3: 3 mg/mL (7.20 mM) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), suspension solution; with ultrasonication. Solubility in Formulation 4: 20 mg/mL (48.03 mM) in 20% SBE-β-CD in Saline (add these co-solvents sequentially from left to right, and one by one), clear solution; with ultrasonication. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.4017 mL | 12.0083 mL | 24.0165 mL | |
| 5 mM | 0.4803 mL | 2.4017 mL | 4.8033 mL | |
| 10 mM | 0.2402 mL | 1.2008 mL | 2.4017 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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