Size | Price | Stock | Qty |
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5mg |
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10mg |
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50mg |
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Other Sizes |
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ln Vitro |
L-690330 (50 μM; 1 hour) enhances p-AMPK and LC3-I/II expression in HEK293 cells and stimulates P-AMPK and autophagy in cells [2].
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ln Vivo |
L-690330 (intracerebroventricular injection; 0.1 μMol) They only lessen the amount of time in the light during the dark/light testing, but otherwise they had no influence on motor activity and coordination during beam walking [3].
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Cell Assay |
Western Blot analysis [2]
Cell Types: HEK293 cells Tested Concentrations: 50 μM Incubation Duration: 1 hour Experimental Results: Increased LC3-I/II and p-AMPK protein levels. |
Animal Protocol |
Animal/Disease Models: Eightweeks old male ICR mice [3]
Doses: 0.1 μmol Route of Administration: Intracerebroventricular injection Experimental Results: ICV injection will not affect the health of mice. |
References |
[1]. Atack JR, et al. In vitro and in vivo inhibition of inositol monophosphatase by the bisphosphonate L-690,330. J Neurochem. 1993 Feb;60(2):652-8.
[2]. Cárdenas C, et al. Essential regulation of cell bioenergetics by constitutive InsP3 receptor Ca2+ transfer to mitochondria.Cell. 2010 Jul 23;142(2):270-83. [3]. Shtein L, et al. The inositol monophosphatase inhibitor L-690,330 affects pilocarpine-behavior and the forced swim test.Psychopharmacology (Berl). 2013 Jun;227(3):503-8. |
Molecular Formula |
C8H12O8P2
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Molecular Weight |
298.12
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Exact Mass |
298.0007
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CAS # |
142523-38-4
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Related CAS # |
L-690330 hydrate
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Appearance |
Typically exists as solids (or liquids in special cases) at room temperature
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SMILES |
OC1C=CC(OC(P(=O)(O)O)(P(=O)(O)O)C)=CC=1
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Synonyms |
L-690330; L 690330; L690330
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HS Tariff Code |
2934.99.9001
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Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment, avoid exposure to moisture. |
Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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Solubility (In Vitro) |
H2O : ~33.33 mg/mL (~111.80 mM)
DMSO : ~25 mg/mL (~83.86 mM) |
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Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (8.39 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (8.39 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C,1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. View More
Solubility in Formulation 3: ≥ 2.5 mg/mL (8.39 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. Solubility in Formulation 4: 100 mg/mL (335.44 mM) in PBS (add these co-solvents sequentially from left to right, and one by one), clear solution; with ultrasonication. |
Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
1 mM | 3.3544 mL | 16.7718 mL | 33.5435 mL | |
5 mM | 0.6709 mL | 3.3544 mL | 6.7087 mL | |
10 mM | 0.3354 mL | 1.6772 mL | 3.3544 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration: mg/mL;
Method for preparing DMSO stock solution: mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation::Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O,mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.