Size | Price | Stock | Qty |
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500mg |
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5g |
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10g |
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Other Sizes |
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ln Vitro |
Levamisole hydrochloride causes neutropenia and agranulocytosis by preventing the formation of white blood cells. Numerous difficulties have been observed in cocaine users due to the growing use of levamisole hydrochloride as an adulterant [1] [2]. Levamisole hydrochloride inhibits most isoforms of alkaline phosphatase, including destructive and placental isoforms, reversibly and non-competitively (e.g., human fetus, kidney, and spleen) [3]. Thus, in biomedical experiments involving detection signal amplification that disrupts phosphatases, like in situ hybridization or Western blotting methods, it is supplied along with substrate to lower background phosphatase activity. C is mounted using it. elegans onto imaging-ready glass slides.
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References |
[1]. Centers for Disease Control and Prevention (CDC). Agranulocytosis associated with cocaine use - four States, March 2008-November 2009.MMWR Morb Mortal Wkly Rep. 2009 Dec 18;58(49):1381-5.
[2]. Zhu NY, et al. Agranulocytosis after consumption of cocaine adulterated with levamisole. Ann Intern Med. 2009 Feb 17;150(4):287-9. [3]. Van Belle H. Alkaline phosphatase. I. Kinetics and inhibition by levamisole of purified isoenzymes from humans. Clin Chem. 1976 Jul;22(7):972-6. [4]. Friedlaender MH, et al. The treatment of herpetic reinfection with levamisole. Am J Ophthalmol. 1978 Aug;86(2):245-9. |
Molecular Formula |
C11H13CLN2S
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Molecular Weight |
240.7523
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CAS # |
16595-80-5
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Related CAS # |
Levamisole-d5 hydrochloride;1246819-64-6;Levamisole;14769-73-4
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Appearance |
Typically exists as solids (or liquids in special cases) at room temperature
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SMILES |
Cl[H].S1C([H])([H])C([H])([H])N2C1=N[C@@]([H])(C1C([H])=C([H])C([H])=C([H])C=1[H])C2([H])[H]
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HS Tariff Code |
2934.99.9001
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Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment, avoid exposure to moisture. |
Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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Solubility (In Vitro) |
H2O : ~50 mg/mL (~207.68 mM)
DMSO : ~15.5 mg/mL (~64.38 mM) Ethanol : ~12.5 mg/mL (~51.92 mM) |
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Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 1.25 mg/mL (5.19 mM) (saturation unknown) in 10% EtOH + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 12.5 mg/mL clear EtOH stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 1.25 mg/mL (5.19 mM) (saturation unknown) in 10% EtOH + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 12.5 mg/mL clear EtOH stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C,1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. View More
Solubility in Formulation 3: ≥ 1.25 mg/mL (5.19 mM) (saturation unknown) in 10% EtOH + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. Solubility in Formulation 4: 100 mg/mL (415.37 mM) in PBS (add these co-solvents sequentially from left to right, and one by one), clear solution; with ultrasonication. |
Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
1 mM | 4.1537 mL | 20.7684 mL | 41.5369 mL | |
5 mM | 0.8307 mL | 4.1537 mL | 8.3074 mL | |
10 mM | 0.4154 mL | 2.0768 mL | 4.1537 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration: mg/mL;
Method for preparing DMSO stock solution: mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation::Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O,mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.