| Size | Price | Stock | Qty |
|---|---|---|---|
| 1mg |
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| 5mg |
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| 10mg |
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| Other Sizes |
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| ln Vitro |
Cardiovascular cell viability can be inhibited by LLL12 (0.01-1 μM; 72 h) in response to cisplatin (0.5 μM, 2.5 μM) and paclitaxel (0.25 μM, 0.5 μM) [1].
|
|---|---|
| ln Vivo |
In mouse osteosarcoma cell and tumor growth, LLL12 (5 mg/kg; intraperitoneal injection; once daily for 13 days) demonstrates potent growth inhibitory action [2].
|
| Cell Assay |
Western blot analysis [1]
Cell Types: A2780, SKOV3, CAOV-3 and OVCAR5 ovarian cancer cell lines Tested Concentrations: 0.25, 0.5 and 1 μM μM) Phosphorylation of STAT3 (Tyr705) in fluorescently labeled dye lines [1]. A2780 and OVCAR5; SKOV3 and CAOV-3 at 1, 2.5 and 5 μM respectively. Incubation Duration: 72 hrs (hours). Experimental Results: Inhibition of STAT3 phosphorylation at Tyr705. |
| Animal Protocol |
Animal/Disease Models: Mouse xenograft SJSA or OS-33 osteosarcoma cells [2]
Doses: 5 mg/kg Route of Administration: intraperitoneal (ip) injection; one time/day for 13 days Experimental Results: Result in OS-33 and SJSA xenograft mice Tumor volume and tumor mass were Dramatically diminished. |
| References |
|
| Molecular Formula |
C14H9NO5S
|
|---|---|
| Molecular Weight |
303.29
|
| Exact Mass |
303.02
|
| CAS # |
1260247-42-4
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| PubChem CID |
46911017
|
| Appearance |
Light yellow to yellow solid powder
|
| LogP |
2.596
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| Hydrogen Bond Donor Count |
2
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| Hydrogen Bond Acceptor Count |
6
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| Rotatable Bond Count |
1
|
| Heavy Atom Count |
21
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| Complexity |
566
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| Defined Atom Stereocenter Count |
0
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| InChi Key |
CQBHSRLUQDYPBU-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C14H9NO5S/c15-21(19,20)10-6-2-4-8-12(10)14(18)7-3-1-5-9(16)11(7)13(8)17/h1-6,16H,(H2,15,19,20)
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| Chemical Name |
5-hydroxy-9,10-dioxoanthracene-1-sulfonamide
|
| Synonyms |
LLL 12; LLL-12; LLL12
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| HS Tariff Code |
2934.99.9001
|
| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
DMSO : ~100 mg/mL (~329.72 mM)
|
|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: 0.5 mg/mL (1.65 mM) in 2% DMSO + 40% PEG300 +5% Tween-80 + 53% Saline (add these co-solvents sequentially from left to right, and one by one), suspension solution; with sonication.
Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 3.2972 mL | 16.4859 mL | 32.9717 mL | |
| 5 mM | 0.6594 mL | 3.2972 mL | 6.5943 mL | |
| 10 mM | 0.3297 mL | 1.6486 mL | 3.2972 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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