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1mg |
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2mg |
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5mg |
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10mg |
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25mg |
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50mg |
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100mg |
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Other Sizes |
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Purity: ≥98%
LY-3475070 (LY3475070; LY 3475070) is a novel, potent, oral and selective CD73 inhibitor (cluster of differentiation 73, IC50 = 28 nM) with potential anticancer activity.
Targets |
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ln Vitro |
With an EC50 of 0.213 μM, CD73-IN-3 suppresses CD73 activity in human serum. At 0.52 μM, 1.56 μM, and 4.68 μM, respectively, CD73-IN-3 suppresses CD73 activity in human serum by 56.8%, 71.7%, and 76.9% [1]. The IC50 of CD73-IN-3 for CD73 is 0.0073 μM, according to the detection results of CD73 in Calu6 human cells [1].
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References |
[1]. Robert Dean Dally, et al. Cd73 inhibitors. Patent WO2019168744 A1.
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Molecular Formula |
C15H18N4O2
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Molecular Weight |
286.335
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Exact Mass |
286.142975
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Elemental Analysis |
C, 62.92; H, 6.34; N, 19.57; O, 11.18
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CAS # |
2375815-63-5
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Appearance |
White to off-white solid
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LogP |
1
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tPSA |
84Ų
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SMILES |
O=C1C(=C([H])N([H])C(N1[H])=O)C1C([H])=C(C(C([H])([H])[H])=NN=1)[C@@]1([H])C([H])([H])[C@]1([H])C([H])(C([H])([H])[H])C([H])([H])[H]
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InChi Key |
WHRUIISQCORGKK-KOLCDFICSA-N
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InChi Code |
InChI=1S/C15H18N4O2/c1-7(2)9-4-11(9)10-5-13(19-18-8(10)3)12-6-16-15(21)17-14(12)20/h5-7,9,11H,4H2,1-3H3,(H2,16,17,20,21)/t9-,11+/m1/s1
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Chemical Name |
5-(5-((1S,2R)-2-isopropylcyclopropyl)-6-methylpyridazin-3-yl)pyrimidine-2,4(1H,3H)-dione
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Synonyms |
LY 3475070 LY-3475070 LY3475070
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HS Tariff Code |
2934.99.9001
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Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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Solubility (In Vitro) |
DMSO : ~100 mg/mL (~349.25 mM)
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Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (8.73 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C,1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (8.73 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly.  (Please use freshly prepared in vivo formulations for optimal results.) |
Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
1 mM | 3.4924 mL | 17.4618 mL | 34.9235 mL | |
5 mM | 0.6985 mL | 3.4924 mL | 6.9847 mL | |
10 mM | 0.3492 mL | 1.7462 mL | 3.4924 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration: mg/mL;
Method for preparing DMSO stock solution: mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation::Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O,mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.