| Size | Price | Stock | Qty |
|---|---|---|---|
| 1mg |
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| 5mg |
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| Other Sizes |
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| ln Vitro |
At concentrations up to 60 μM, MB710 (0-200 μM; 72 hours) shown comparatively minimal toxicity against all cell lines examined, but at higher concentrations, it first showed signs of decreasing viability [1]. IC50 values of 90, 120, >120, and >120 μM for the cancer cell lines NugC3, NugC4, WI38, and SW1088 were obtained after treatment [1]. MB710 (0-120 μM) in p53-Y220C for 72 hours in HUH-7 cells
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|---|---|
| Cell Assay |
cell viability assay [1]
Cell Types: NUGC3 (mutant p53 Y220C), HUH-7 (mutant p53 Y220C), NUGC4 (p53 WT), Exhibits stronger cytotoxic effects in the presence of HUH-6 [1]. Cells (p53 WT) Tested Concentrations: 0-200 μM Incubation Duration: 72 hrs (hours) Experimental Results: Relatively low toxicity was shown for all cell lines tested at concentrations up to 60 μM. NUGC3 was the most sensitive cell line. |
| References |
| Molecular Formula |
C16H16IN3O3S
|
|---|---|
| Molecular Weight |
457.286014556885
|
| Exact Mass |
456.995
|
| CAS # |
2230044-57-0
|
| PubChem CID |
133082032
|
| Appearance |
Light yellow to green yellow solid powder
|
| LogP |
4.3
|
| Hydrogen Bond Donor Count |
2
|
| Hydrogen Bond Acceptor Count |
6
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| Rotatable Bond Count |
5
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| Heavy Atom Count |
24
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| Complexity |
462
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| Defined Atom Stereocenter Count |
0
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| SMILES |
IC1C(=C(C(=O)O)C2=C(C=1N1C=CC=C1)SC(=N2)N(CC)CC)O
|
| InChi Key |
SEBJATZOIHUGAU-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C16H16IN3O3S/c1-3-19(4-2)16-18-11-9(15(22)23)13(21)10(17)12(14(11)24-16)20-7-5-6-8-20/h5-8,21H,3-4H2,1-2H3,(H,22,23)
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| Chemical Name |
2-(diethylamino)-5-hydroxy-6-iodo-7-pyrrol-1-yl-1,3-benzothiazole-4-carboxylic acid
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| Synonyms |
MB710; MB 710; MB-710
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
DMSO : ~16.67 mg/mL (~36.45 mM)
|
|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: 1.25 mg/mL (2.73 mM) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), suspension solution; with sonication.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 12.5 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: 1.25 mg/mL (2.73 mM) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), suspension solution; with ultrasonication. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 12.5 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.1868 mL | 10.9340 mL | 21.8680 mL | |
| 5 mM | 0.4374 mL | 2.1868 mL | 4.3736 mL | |
| 10 mM | 0.2187 mL | 1.0934 mL | 2.1868 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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