| Size | Price | Stock | Qty |
|---|---|---|---|
| 5mg |
|
||
| 10mg |
|
||
| 50mg |
|
||
| Other Sizes |
|
| ln Vitro |
Mecamylamine hydrochloride (0.5–9 μM, bath spray) enhances the frequency of fluorescence in 5-HT DRN (dorsal raphe nucleus) neurons that have been found [1]. Mecamylamine hydrochloride (0.5–9 μM, bath spray) increases glutamatergic input to 5-HT DRN neurons while decreasing GABAergic input [1]. In rabbit main rotary artery sections, mecamylamine hydrochloride (1 mM, 5 min) burns histamine and causes histamine-induced contraction [2]. Nicotine's neuroprotective properties are affected by mecamylamine hydrochloride (10 μM, 48 h) [3]. In HDMVEC, mecamylamine hydrochloride (1–100 nM, 30 minutes) reduces endothelial tube formation in a dose-dependent manner [4]. 3]
|
|---|---|
| ln Vivo |
In a model of choroidal neovascularization (CNV), mecamylamine hydrochloride (subcutaneous pump, 50 mg/kg/day, 2 days) reduces CNV [4]. Mecamylamine hydrochloride (ip, 0.5–1 mg/kg) exhibits β2- and α7-subunit-dependent antidepressant-like effects in the tail suspension test (TST) and forced swim test (FST) in C57BL/6J mice [ 5].
|
| Cell Assay |
Western Blot Analysis[3]
Cell Types: SCG Neuronal Tested Concentrations: 10 μM Incubation Duration: 48 hrs (hours) Experimental Results: diminished nicotine-promoted increase in ERK1/2. |
| Animal Protocol |
Animal/Disease Models: Choroidal neovascularization (CNV) mouse model [1] Usage and
Doses: 50 mg/kg/day, 2 days of Route of Administration: A subcutaneouspump is implanted under the back skin, 200 μL, and the average pump speed is 0.5 μL/h. Experimental Results: In the absence of nicotine, the development of CNV at the site of Bruch's membrane rupture was inhibited. Animal/Disease Models: C57BL/6J mice [5] Doses: 0.5-1 mg/kg Route of Administration: intraperitoneal (ip) injection Experimental Results: No effect on β2 gene knockout mice and α7 gene knockout mice, but diminished wild-type littermates Mouse immobility time in FST. |
| References |
[1]. Omar Hernández-González, et al. Mechanisms of stimulatory effects of mecamylamine on the dorsal raphe neurons. Brain Res Bull. 2020 Nov;164:289-298.
[2]. C P Robinson, et al. The influence of mecamylamine on contractions induced by different agonists and on the role of calcium ions in the isolated rabbit aorta. J Pharmacol Exp Ther. 1976 Apr;197(1):57-65. [3]. Mahadevappa P Badanavalu, et al. Nicotine is neuroprotective to neonatal neurons of sympathetic ganglion in rat. Auton Neurosci. 2019 Jan;216:25-32. [4]. Katsuji Kiuchi, et al. Mecamylamine suppresses Basal and nicotine-stimulated choroidal neovascularization. Invest Ophthalmol Vis Sci. 2008 Apr;49(4):1705-11. [5]. Rabenstein RL, et al. The nicotinic antagonist mecamylamine has antidepressant-like effects in wild-type but not beta2- or alpha7-nicotinic acetylcholine receptor subunit knockout mice. Psychopharmacology (Berl). 2006 Dec;189(3):395-401. |
| Molecular Formula |
C11H22CLN
|
|---|---|
| Molecular Weight |
203.75
|
| Exact Mass |
203.1441
|
| CAS # |
826-39-1
|
| Related CAS # |
Mecamylamine;60-40-2;Mecamylamine hydrochloride-13C4,15N
|
| Appearance |
Typically exists as solids (or liquids in special cases) at room temperature
|
| SMILES |
CC1(C)C2CCC(C2)C1(C)NC.Cl
|
| Synonyms |
Mevasin; Mecamine hydrochloride Mecamylamine HCl; Inversine
|
| HS Tariff Code |
2934.99.9001
|
| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment, avoid exposure to moisture. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
H2O : ~100 mg/mL (~490.80 mM)
DMSO : ~31.25 mg/mL (~153.37 mM) |
|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.08 mg/mL (10.21 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.08 mg/mL (10.21 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. View More
Solubility in Formulation 3: ≥ 2.08 mg/mL (10.21 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 4.9080 mL | 24.5399 mL | 49.0798 mL | |
| 5 mM | 0.9816 mL | 4.9080 mL | 9.8160 mL | |
| 10 mM | 0.4908 mL | 2.4540 mL | 4.9080 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
Products are for research use only; We do not sell to patients
Copyright 2020 InvivoChem LLC | All Rights Reserved
