| Size | Price | Stock | Qty |
|---|---|---|---|
| 5mg |
|
||
| 10mg |
|
||
| 25mg |
|
||
| 50mg |
|
||
| 100mg |
|
||
| 250mg |
|
||
| 500mg |
|
||
| Other Sizes |
|
| ln Vitro |
Meisoindigo (Dian III; 5–20 M; for 24 hours) prevents AML cell lines from proliferating[1].
Meisoindigo (10 μM; for 24 hours) induces apoptosis of acute myeloid leukemia[1]. Meisoindigo (5-10 μM; for 24 hours) causes cell-cycle arrest[1]. Meisoindigo (5-10 μM; for 24 hours) elevates pro-apoptotic Bak and cleaved caspase-3 while lowering Bcl-2 and Bcl-xL levels in HL-60 cells[1]. Meisoindigo (10, 30, 50, 100, 150 μM; 24 hours) blocks NLRP3 inflammasome activation and M1/M2 polarization brought on by LPS (1 μg/mL) in HT-22 and BV2 cells by down-regulating TLR4 pathways after OGD/R[2]. |
|---|---|
| ln Vivo |
Meisoindigo (Dian III; 50–150 mg/kg; IP; daily; for 14 days) has anti-leukemic activity in vivo[1].
Meisoindigo significantly reduces infarct volume and improves neurological deficits 3 days after middle cerebral artery occlusion (MCAO) in Wild-type C57BL/6J mice (25-30 g) when administered intravenously (2, 4, 8, 12 mg/kg) prior to MCAO and 2 hours after reperfusion. Meisoindigo decreases brain edema and AQP4 expression[2]. |
| Animal Protocol |
HL-60, NB4, U937 leukemic cell lines
5, 10, 15, 20 μM IP; daily; for 14 days |
| References |
|
| Molecular Formula |
C18H14N2O2
|
|---|---|
| Molecular Weight |
290.31
|
| Exact Mass |
290.10553
|
| Elemental Analysis |
C, 74.47; H, 4.86; N, 9.65; O, 11.02
|
| CAS # |
97207-47-1
|
| Related CAS # |
97207-47-1
|
| Appearance |
Red solid powder
|
| SMILES |
CN1C2=CC=CC=C2C(=C1O)C3=C4C=CC=CC4=NC3=O
|
| InChi Key |
XZYXCQXTKOYHGK-UHFFFAOYSA-N
|
| InChi Code |
InChI=1S/C17H12N2O2/c1-19-13-9-5-3-7-11(13)15(17(19)21)14-10-6-2-4-8-12(10)18-16(14)20/h2-9,21H,1H3
|
| Chemical Name |
3-(2-hydroxy-1-methylindol-3-yl)indol-2-one
|
| Synonyms |
Meisoindigo; N-Methylisoindigotin; Methyl isoindigotin
|
| HS Tariff Code |
2934.99.9001
|
| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
DMSO: ~55 mg/mL (~199.1 mM)
Ethanol: ~1 mg/mL (~3.6 mM) |
|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (9.05 mM) (saturation unknown) in 10% DMSO + 40% PEG300 +5% Tween-80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 + to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 3.4446 mL | 17.2230 mL | 34.4459 mL | |
| 5 mM | 0.6889 mL | 3.4446 mL | 6.8892 mL | |
| 10 mM | 0.3445 mL | 1.7223 mL | 3.4446 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
Products are for research use only; We do not sell to patients
Copyright 2020 InvivoChem LLC | All Rights Reserved
