Size | Price | Stock | Qty |
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50mg |
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100mg |
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250mg |
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500mg |
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1g |
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Other Sizes |
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Purity: ≥98%
Mestranol (Ethinyl Estradiol 3 Methyl Ether; Devocin; Norquen; Ovastol), the 3-methyl ether of ethinyl estradiol, is a potent estrogen receptor agonist that has been used as an oral contraceptive. Mestranol is a prodrug of ethinylestradiol and is biologically inactive. It has to be activated through demethylation in the liver.
ln Vitro |
Mestranol, a synthetic estrogen with modest potency, has demonstrated much greater stability in hepatoma cell culture as compared to 17β-Estradiol [3]. Over a period of six days, mestranol (10 μM) can boost the proliferation of ERpositive MCF-7 WS8 cells up to 250% over control levels. Tamoxifen can partially reverse this growth stimulation. Mestranol (10 μM; 6 days) on Hep G2 hepatoma cells, however, reduces the development of Hep 3B cells by 40% in comparison to control cells. It is possible to stop cell proliferation using mestranol alone or in combination with tamoxifen. Additionally, tamoxifen and cotreatment have a cumulative effect on growth inhibition[2].
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ln Vivo |
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Animal Protocol |
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References |
[1]. H Kappus, et al. Affinity of ethynyl-estradiol and mestranol for the uterine estrogen receptor and for the microsomal mixed function oxidase of the liver. J Steroid Biochem. 1973 Mar;4(2):121-8.
[2]. J W Goldzieher, et al. Pharmacokinetics of ethinyl estradiol and mestranol. Am J Obstet Gynecol. 1990 Dec;163(6 Pt 2):2114-9. [3]. S Y Jiang, et al. Tamoxifen inhibits hepatoma cell growth through an estrogen receptor independent mechanism. J Hepatol. 1995 Dec;23(6):712-9. |
Molecular Formula |
C21H26O2
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Molecular Weight |
310.43
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CAS # |
72-33-3
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Related CAS # |
Mestranol-d2;Mestranol-d4
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Appearance |
Typically exists as solids (or liquids in special cases) at room temperature
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SMILES |
C[C@@]1([C@]2(O)C#C)[C@](CC2)([H])[C@@](CCC3=C4C=CC(OC)=C3)([H])[C@]4([H])CC1
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InChi Key |
IMSSROKUHAOUJS-UHFFFAOYSA-N
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InChi Code |
InChI=1S/C21H26O2/c1-4-21(22)12-10-19-18-7-5-14-13-15(23-3)6-8-16(14)17(18)9-11-20(19,21)2/h1,6,8,13,17-19,22H,5,7,9-12H2,2-3H3
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Chemical Name |
17-ethynyl-3-methoxy-13-methyl-7,8,9,11,12,13,14,15,16,17-decahydro-6H-cyclopenta[a]phenanthren-17-ol
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Synonyms |
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HS Tariff Code |
2934.99.9001
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Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
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Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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Solubility (In Vitro) |
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Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.08 mg/mL (6.70 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C,1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. Solubility in Formulation 2: ≥ 2.08 mg/mL (6.70 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly.  (Please use freshly prepared in vivo formulations for optimal results.) |
Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
1 mM | 3.2213 mL | 16.1067 mL | 32.2134 mL | |
5 mM | 0.6443 mL | 3.2213 mL | 6.4427 mL | |
10 mM | 0.3221 mL | 1.6107 mL | 3.2213 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration: mg/mL;
Method for preparing DMSO stock solution: mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation::Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O,mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.