Size | Price | Stock | Qty |
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250mg |
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500mg |
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1g |
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Other Sizes |
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Methicillin sodium salt (also known as Meticillin sodium) is a β-lactam antibiotic of the penicillin class. It acts by inhibiting penicillin-binding proteins that are involved in the synthesis of peptidoglycan. Compared to other penicillins that face antimicrobial resistance due to β-lactamase, it is less active, can be administered only parenterally, and has a higher frequency of interstitial nephritis, an otherwise-rare adverse effect of penicillins. However, selection of methicillin depended on the outcome of susceptibility testing of the sampled infection, and since it is no longer produced, it is also not routinely tested for any more. It also served a purpose in the laboratory to determine the antibiotic sensitivity of Staphylococcus aureus to other penicillins facing β-lactam resistance; this role has now been passed on to other penicillins, namely cloxacillin, as well as genetic testing for the presence of mecA gene by PCR.
Targets |
β-lactam
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ln Vitro |
Methicillin sodium salt (100 μg/mL; 18 h) incubates intracellularly for 18 hours before killing S. aureus[1].
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ln Vivo |
Methicillin sodium salt (42.5 and 85 mg/kg) against enterococcus in leukocytes, administered intramuscularly four times a day for 21 days or until the cells die naturally[1].
Mice infected with methicillin sodium salt (400 mg/kg; intraperitoneally once) fare better than the comparison group[2]. Mice with infection cannot die if methicillin sodium salt (400 mg/kg; i.h. once) is administered[2]. |
Cell Assay |
Cell Line: Leukocytes
Concentration: 100 μg/mL Incubation Time: 18 hours Result: demonstrated superior results in an aerobic environment and successfully eradicated S. aureus at a 3.13 μg/mL bactericidal concentration. |
Animal Protocol |
Animal Model: White New Zealand rabbits, weighing 2.0 to 3.0 kg[1]
Dosage: 42.5 and 85 mg/kg Administration: Intramuscular injection; 42.5 and 85 mg/kg four times daily; for 21 days or till spontaneous death Result: Inhibited enterococcus at 24 hours with a half-live of 1.1 h, but showed no statistical significance to rabbits. |
References |
Molecular Formula |
C17H19N2O6S-.NA+
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Molecular Weight |
402.39736
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CAS # |
132-92-3
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Related CAS # |
Methicillin;61-32-5;Methicillin sodium hydrate;7246-14-2
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Appearance |
Typically exists as solids (or liquids in special cases) at room temperature
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SMILES |
[O-]C([C@@H](C(C)(C)S[C@]1([H])[C@@H]2NC(C3=C(OC)C=CC=C3OC)=O)N1C2=O)=O.[Na+]
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InChi Key |
MGFZNWDWOKASQZ-UMLIZJHQSA-M
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InChi Code |
InChI=1S/C17H20N2O6S.Na/c1-17(2)12(16(22)23)19-14(21)11(15(19)26-17)18-13(20)10-8(24-3)6-5-7-9(10)25-4;/h5-7,11-12,15H,1-4H3,(H,18,20)(H,22,23);/q;+1/p-1/t11-,12+,15-;/m1./s1
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Chemical Name |
sodium;(2S,5R,6R)-6-[(2,6-dimethoxybenzoyl)amino]-3,3-dimethyl-7-oxo-4-thia-1-azabicyclo[3.2.0]heptane-2-carboxylate
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Synonyms |
Methicillin sodium salt
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HS Tariff Code |
2934.99.9001
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Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment, avoid exposure to moisture. |
Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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Solubility (In Vitro) |
DMSO : ~125 mg/mL (~310.64 mM )
H2O : ~100 mg/mL (~248.51 mM ) |
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Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.08 mg/mL (5.17 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.08 mg/mL (5.17 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C,1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. View More
Solubility in Formulation 3: ≥ 2.08 mg/mL (5.17 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. Solubility in Formulation 4: 10% DMSO+40% PEG300+5% Tween-80+45% Saline: ≥ 2.08 mg/mL (5.17 mM) Solubility in Formulation 5: 100 mg/mL (248.51 mM) in PBS (add these co-solvents sequentially from left to right, and one by one), clear solution; with ultrasonication. |
Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
1 mM | 2.4851 mL | 12.4254 mL | 24.8509 mL | |
5 mM | 0.4970 mL | 2.4851 mL | 4.9702 mL | |
10 mM | 0.2485 mL | 1.2425 mL | 2.4851 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration: mg/mL;
Method for preparing DMSO stock solution: mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation::Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O,mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.