| Size | Price | Stock | Qty |
|---|---|---|---|
| 5mg |
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| 10mg |
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| 50mg |
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| 100mg |
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| Other Sizes |
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| ln Vitro |
MK6-83 does not show enough cytotoxicity between 0.2 and 30 μM [1]. When it comes to fibroblast lysosomes isolated from cells expressing R403C or V446L, MK6-83 seems to be much more powerful than when it comes to cells isolated from TRPML1 −/− cells [1].
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|---|---|
| Cell Assay |
Cell viability assay[1].
Cell Types: Lysosomes isolated from fibroblast cell lines of MLIV patients carrying F408D, R403C or V446L mutations[1]. Tested Concentrations: 0-10μM. Incubation Duration: 24 hrs (hours). Experimental Results: Effective on fibroblast lysosomes isolated from R403C or V446L expressing cells. There was no significant effect on lysosomes isolated from TRPML1-/- fibroblasts. |
| References |
| Molecular Formula |
C16H20N2O2S2
|
|---|---|
| Molecular Weight |
336.46
|
| Exact Mass |
336.096
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| CAS # |
1062271-24-2
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| PubChem CID |
18191179
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| Appearance |
Light yellow to yellow solid powder
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| Density |
1.3±0.1 g/cm3
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| Boiling Point |
502.6±60.0 °C at 760 mmHg
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| Flash Point |
257.8±32.9 °C
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| Vapour Pressure |
0.0±1.3 mmHg at 25°C
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| Index of Refraction |
1.633
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| LogP |
3.4
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| Hydrogen Bond Donor Count |
1
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| Hydrogen Bond Acceptor Count |
5
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| Rotatable Bond Count |
4
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| Heavy Atom Count |
22
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| Complexity |
457
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| Defined Atom Stereocenter Count |
0
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| InChi Key |
IRGYSXZCDAWOOC-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C16H20N2O2S2/c1-13-9-10-16(21-13)22(19,20)17-14-7-3-4-8-15(14)18-11-5-2-6-12-18/h3-4,7-10,17H,2,5-6,11-12H2,1H3
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| Chemical Name |
5-methyl-N-(2-piperidin-1-ylphenyl)thiophene-2-sulfonamide
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| Synonyms |
MK683; MK6 83; MK6-83
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ~25 mg/mL (~74.30 mM)
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|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (7.43 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: 2.5 mg/mL (7.43 mM) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), suspension solution; with ultrasonication. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.9721 mL | 14.8606 mL | 29.7212 mL | |
| 5 mM | 0.5944 mL | 2.9721 mL | 5.9442 mL | |
| 10 mM | 0.2972 mL | 1.4861 mL | 2.9721 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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