| Size | Price | Stock | Qty |
|---|---|---|---|
| 5mg |
|
||
| 10mg |
|
||
| 25mg |
|
||
| 50mg |
|
||
| 100mg |
|
||
| 250mg |
|
||
| 500mg |
|
||
| Other Sizes |
|
Purity: ≥98%
ML141 (also called CID-2950007; ML-141; CID2950007; ML 141) is a novel potent, selective and reversible non-competitive inhibitor of Rho family GTPase cdc42 with potential anticancer activity. With an IC50 of 200 nM, it suppresses CDC42. By causing both cell death and division inhibition, ML141 improves TMX's capacity to inhibit the growth of BLBC cells. A substantial defense against metformin-induced apoptosis is provided by ML141 to neuroblastoma cells. In addition, ML141 reduces K. pneumoniae invasion in a manner that is dependent on dosage.
| Targets |
cdc42 (IC50 = 200 nM)
|
|---|---|
| ln Vitro |
ML141 (CID-2950007) is not cytotoxic in either cell line at doses of 0.1-3 μM after treatment for 4 days. Ten milligrams of the compound did not cause any cytotoxicity in OVCA429 cells, but after four days of treatment, SKOV3ip cells showed some cytotoxicity at this concentration, though not to the point of statistical significance. When applied at 10 μM for 24 or 48 hours, respectively, ML141 does not cause any harm to Swiss 3T3 or Vero E6 cells[1].
ML141 suppresses the migration of ovarian cancer cells and prevents the formation of 3T3 fibroblast filopodia[1]. |
| ln Vivo |
ML141 (CID-2950007) (10 μM; intracerebroventricular injection) induces acute anxiety in mice[3].
|
| Enzyme Assay |
Overnight at 4°C, wild-type GST-Cdc42 (4 μM) is bound to GSH-beads. By incubating with a buffer containing 10 mM EDTA for 20 minutes at 30°C, washing twice with NP-HPS buffer, and then re-suspending in the same buffer containing 1 mM EDTA/or 1 mM MgCl2, 1 mM DTT, and 0.1% BSA, Cdc42 on GSH-beads is nucleotide-depleted. A 15-minute RT incubation of the protein-bead complex blocks Cdc42 unbound sites. After incubating 30 μL of this suspension with a 20 mM inhibitor for three minutes at room temperature, 30 μL of different concentrations of ice-cold BODIPY-FL-GTP are added. Samples are incubated for 45 minutes at 4° C, and an Accuri flow cytometer is used to measure the binding of fluorescent nucleotide to enzyme. The program GraphPad Prism is used to export and plot raw data.
|
| Cell Assay |
The adherent cell CeligoTM cytometer is used to count the number of live and dead cells, which are indicated by positive Calcein-AM and PI staining, respectively, after the cells are incubated with 500 nM Calcein-AM and 1 µM PI for 15 minutes.
|
| Animal Protocol |
NOD/SCID mice bearing MDA-MB 231 derived tumors
1 mg/day i.p. |
| References | |
| Additional Infomation |
4-[3-(4-methoxyphenyl)-5-phenyl-3,4-dihydropyrazol-2-yl]benzenesulfonamide is a sulfonamide.
|
| Molecular Formula |
C22H21N3O3S
|
|
|---|---|---|
| Molecular Weight |
407.49
|
|
| Exact Mass |
407.13
|
|
| Elemental Analysis |
C, 64.85; H, 5.19; N, 10.31; O, 11.78; S, 7.87
|
|
| CAS # |
71203-35-5
|
|
| Related CAS # |
|
|
| PubChem CID |
2950007
|
|
| Appearance |
White to off-white solid powder
|
|
| Density |
1.3±0.1 g/cm3
|
|
| Boiling Point |
622.9±65.0 °C at 760 mmHg
|
|
| Melting Point |
216 °C
|
|
| Flash Point |
330.5±34.3 °C
|
|
| Vapour Pressure |
0.0±1.8 mmHg at 25°C
|
|
| Index of Refraction |
1.652
|
|
| LogP |
1.82
|
|
| Hydrogen Bond Donor Count |
1
|
|
| Hydrogen Bond Acceptor Count |
6
|
|
| Rotatable Bond Count |
5
|
|
| Heavy Atom Count |
29
|
|
| Complexity |
668
|
|
| Defined Atom Stereocenter Count |
0
|
|
| SMILES |
S(C1C([H])=C([H])C(=C([H])C=1[H])N1C([H])(C2C([H])=C([H])C(=C([H])C=2[H])OC([H])([H])[H])C([H])([H])C(C2C([H])=C([H])C([H])=C([H])C=2[H])=N1)(N([H])[H])(=O)=O
|
|
| InChi Key |
QBNZBMVRFYREHK-UHFFFAOYSA-N
|
|
| InChi Code |
InChI=1S/C22H21N3O3S/c1-28-19-11-7-17(8-12-19)22-15-21(16-5-3-2-4-6-16)24-25(22)18-9-13-20(14-10-18)29(23,26)27/h2-14,22H,15H2,1H3,(H2,23,26,27)
|
|
| Chemical Name |
4-[3-(4-methoxyphenyl)-5-phenyl-3,4-dihydropyrazol-2-yl]benzenesulfonamide
|
|
| Synonyms |
|
|
| HS Tariff Code |
2934.99.9001
|
|
| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
|
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
|
|||
|---|---|---|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (6.14 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly. Solubility in Formulation 2: 2.08 mg/mL (5.10 mM) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), suspension solution; with ultrasonication. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. View More
Solubility in Formulation 3: ≥ 2.08 mg/mL (5.10 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. Solubility in Formulation 4: 2% DMSO +30% PEG 300 +5% Tween 80 +ddH2O: 10mg/mL |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.4540 mL | 12.2702 mL | 24.5405 mL | |
| 5 mM | 0.4908 mL | 2.4540 mL | 4.9081 mL | |
| 10 mM | 0.2454 mL | 1.2270 mL | 2.4540 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
|
Products are for research use only; We do not sell to patients
Copyright 2020 InvivoChem LLC | All Rights Reserved
COA
