| Size | Price | Stock | Qty |
|---|---|---|---|
| 1mg |
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| 5mg |
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| 10mg |
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| Other Sizes |
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| ln Vitro |
Naphthylfluorescein (compound 19) inhibits HIF-1 reporter gene activity in a concentration-dependent manner (0-10 μM; 24 hours). [1]. Comparing MMI-0100 (0.25 and 0.5 mM; 24 hours) to control cells treated with 20 ng/ml TNF-α alone, there was a small increase in cell proliferation in both cell types [1]. In comparison to controls, MMI-0100 (1 mM) treatment also boosted EC (11%) and SMC (7%) proliferation; however, this reaction was not as pronounced as that caused by MMI-0100 0.5 mM treatment [1]. At any dose, MMI-0100 does not cause EC apoptosis [1].
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|---|---|
| ln Vivo |
In a mouse vein graft model, MMI-0100 (100 μM; 28 days) inhibits intimal hyperplasia [1].
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| Cell Assay |
Cell Viability Assay[1]
Cell Types: Human Endothelial Cells (EC) and Smooth Muscle Cells (SMC) Tested Concentrations: 0.25, 0.5 and 1 mM Incubation Duration: 24 hrs (hours) Experimental Results: Cell proliferation was slightly higher in both cell types compared to control cells There is an increase. |
| Animal Protocol |
Animal/Disease Models: 12weeks old C57Bl/6 wild-type mice (intimal hyperplasia) [1]
Doses: 100 μM Route of Administration: vein grafts, 28 days Experimental Results: All vein grafts treated with MMI-0100 Wall thickness diminished at all time points, with the control graft thickening 2.6-fold at 4 weeks compared to 4.7-fold at 4 weeks. |
| References |
| Molecular Formula |
C98H171N37O26
|
|---|---|
| Molecular Weight |
2283.64
|
| Exact Mass |
2283.322
|
| CAS # |
1039342-24-9
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| PubChem CID |
127043567
|
| Appearance |
White to off-white solid powder
|
| LogP |
-9.7
|
| Hydrogen Bond Donor Count |
39
|
| Hydrogen Bond Acceptor Count |
32
|
| Rotatable Bond Count |
80
|
| Heavy Atom Count |
161
|
| Complexity |
5010
|
| Defined Atom Stereocenter Count |
21
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| SMILES |
C(O)(=O)[C@H](C)NC(=O)[C@H](C)NC(=O)[C@H](C(C)C)NC(=O)CNC(=O)[C@H](CC(C)C)NC(=O)[C@H](CCC(N)=O)NC(=O)[C@H](CCCNC(N)=N)NC(=O)[C@H](C)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](C)NC(=O)[C@H](CCCCN)NC(=O)[C@H](C)NC(=O)[C@H](CCCNC(N)=N)NC(=O)[C@H](C)NC(=O)[C@H](CCC(N)=O)NC(=O)[C@H](CCCNC(N)=N)NC(=O)[C@H](C)NC(=O)[C@H](C)NC(=O)[C@H](C)NC(=O)[C@H](CCCNC(N)=N)NC(=O)[C@H](C)NC(=O)[C@H](CC1=CC=C(O)C=C1)N
|
| InChi Key |
NXUWTKIOMJSLSV-DEEZXRHXSA-N
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| InChi Code |
InChI=1S/C98H171N37O26/c1-45(2)41-68(84(150)115-44-72(139)135-73(47(5)6)93(159)124-50(9)76(142)125-57(16)94(160)161)134-91(157)67(33-35-71(102)138)132-90(156)65(27-22-40-114-98(109)110)130-81(147)55(14)123-92(158)69(42-46(3)4)133-82(148)56(15)121-85(151)61(23-17-18-36-99)126-79(145)53(12)120-87(153)63(25-20-38-112-96(105)106)128-80(146)54(13)122-88(154)66(32-34-70(101)137)131-89(155)64(26-21-39-113-97(107)108)129-77(143)51(10)117-74(140)48(7)116-75(141)49(8)119-86(152)62(24-19-37-111-95(103)104)127-78(144)52(11)118-83(149)60(100)43-58-28-30-59(136)31-29-58/h28-31,45-57,60-69,73,136H,17-27,32-44,99-100H2,1-16H3,(H2,101,137)(H2,102,138)(H,115,150)(H,116,141)(H,117,140)(H,118,149)(H,119,152)(H,120,153)(H,121,151)(H,122,154)(H,123,158)(H,124,159)(H,125,142)(H,126,145)(H,127,144)(H,128,146)(H,129,143)(H,130,147)(H,131,155)(H,132,156)(H,133,148)(H,134,157)(H,135,139)(H,160,161)(H4,103,104,111)(H4,105,106,112)(H4,107,108,113)(H4,109,110,114)/t48-,49-,50-,51-,52-,53-,54-,55-,56-,57-,60-,61-,62-,63-,64-,65-,66-,67-,68-,69-,73-/m0/s1
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| Chemical Name |
(2S)-2-[[(2S)-2-[[(2S)-2-[[2-[[(2S)-2-[[(2S)-5-amino-2-[[(2S)-2-[[(2S)-2-[[(2S)-2-[[(2S)-2-[[(2S)-6-amino-2-[[(2S)-2-[[(2S)-2-[[(2S)-2-[[(2S)-5-amino-2-[[(2S)-2-[[(2S)-2-[[(2S)-2-[[(2S)-2-[[(2S)-2-[[(2S)-2-[[(2S)-2-amino-3-(4-hydroxyphenyl)propanoyl]amino]propanoyl]amino]-5-carbamimidamidopentanoyl]amino]propanoyl]amino]propanoyl]amino]propanoyl]amino]-5-carbamimidamidopentanoyl]amino]-5-oxopentanoyl]amino]propanoyl]amino]-5-carbamimidamidopentanoyl]amino]propanoyl]amino]hexanoyl]amino]propanoyl]amino]-4-methylpentanoyl]amino]propanoyl]amino]-5-carbamimidamidopentanoyl]amino]-5-oxopentanoyl]amino]-4-methylpentanoyl]amino]acetyl]amino]-3-methylbutanoyl]amino]propanoyl]amino]propanoic acid
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture and light. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
H2O : ~100 mg/mL (~43.79 mM)
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|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: 14.29 mg/mL (6.26 mM) in PBS (add these co-solvents sequentially from left to right, and one by one), clear solution; with sonication.
(Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 0.4379 mL | 2.1895 mL | 4.3790 mL | |
| 5 mM | 0.0876 mL | 0.4379 mL | 0.8758 mL | |
| 10 mM | 0.0438 mL | 0.2189 mL | 0.4379 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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