Size | Price | Stock | Qty |
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5mg |
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10mg |
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25mg |
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50mg |
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100mg |
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250mg |
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500mg |
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Purity: ≥98%
MPTP hydrochloride is a dopaminergic neurotoxin and cause selective destruction of dopaminergic neurons in animal models of parkinsonism. MPTP prevented ICR mice's (20–30 g) phrenic nerve–hemidiaphragm preparations from exhibiting nerve-evoked twitches. MPTP, TC, and PP all had twitch inhibition IC50 values of 53, 0.7, and 123 uM, respectively.
ln Vitro |
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ln Vivo |
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Enzyme Assay |
MPTP prevented phrenic nerve-hemidiaphragm preparations from ICR mice (20–30 g) from experiencing nerve-evoked twitches. The MPTP's inhibitory effect was amplified by PP 50 uM but not by PA 50 uM or TP 50 uM. The amplitude of twitch was inhibited by PP 100 uM alone. The MPTP, TC, and PP twitch inhibition IC50 values were 53, 0.7, and 123 uM, in that order. MPTP and TC had IC50 values of 18 and 0.3 uM, respectively, after pretreatment with PP 50 uM.
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Cell Assay |
N2AB-1 and C6 glioma cells were plated using the culture medium previously mentioned in 24-well costar dishes (16 mm diameter), with 50,000 cells per well. Following a 24-hour period, duplicate medium containing different MPTP or MPP+ concentrations was added. After three days of treatment, both control and treated cells were trypsinized and their counts were measured using a hemocytometer each day.
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Animal Protocol |
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References |
Molecular Formula |
C12H15N.HCL
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Molecular Weight |
209.72
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Exact Mass |
209.1
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CAS # |
23007-85-4
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Related CAS # |
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Appearance |
Powder
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SMILES |
CN1CCC(=CC1)C2=CC=CC=C2.Cl
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InChi Key |
KOWJANGMTAZWDT-UHFFFAOYSA-N
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InChi Code |
InChI=1S/C12H15N.ClH/c1-13-9-7-12(8-10-13)11-5-3-2-4-6-11;/h2-7H,8-10H2,1H3;1H
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Chemical Name |
1-methyl-4-phenyl-3,6-dihydro-2H-pyridine;hydrochloride
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Synonyms |
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HS Tariff Code |
2934.99.9001
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Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment, avoid exposure to moisture. |
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Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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Solubility (In Vitro) |
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Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 1.67 mg/mL (7.96 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 16.7 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 1.67 mg/mL (7.96 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 16.7 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C,1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. View More
Solubility in Formulation 3: ≥ 1.67 mg/mL (7.96 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. Solubility in Formulation 4: 5%DMSO+ 40%PEG300+ 5%Tween 80+ 50%ddH2O: 2.1mg/ml (10.01mM) Solubility in Formulation 5: ≥ 100 mg/mL (476.83 mM) (saturation unknown) in PBS (add these co-solvents sequentially from left to right, and one by one), clear solution. |
Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
1 mM | 4.7683 mL | 23.8413 mL | 47.6826 mL | |
5 mM | 0.9537 mL | 4.7683 mL | 9.5365 mL | |
10 mM | 0.4768 mL | 2.3841 mL | 4.7683 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration: mg/mL;
Method for preparing DMSO stock solution: mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation::Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O,mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.