Size | Price | Stock | Qty |
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1g |
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2g |
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5g |
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10g |
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Other Sizes |
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Purity: ≥98%
MTT (Thiazolyl Blue; Tetrazolium Bromide; Methylthiazolyldiphenyl-tetrazolium bromide), a colorimetric agent, is a cell-permeable and positively charged tetrazolium dye that is widely used to measure cell proliferation. Its mechanism of action is to detect reductive metabolism in cells. MTT is reduced from yellow color to purple formazan in living cells. MTT is used in conjunction with rhodamine B to measure mitochondrial membrane potential. MTT-formazan is generated in mitochondria acts as a fluorescence quencher for rhodamine that distributes across membranes of viable cells according to membrane potential. In the absence of mPMS, cellular reduction of MTT is strong. MTT is membrane-impermeable when incorporated into large unilamellar liposomes, and therefore it is taken up by cells via endocytosis. However, it can be taken up by cells in the free form through the plasma membrane and then reduced to formazan by intracellular NAD(P)H-oxidoreductases. It is frequently used in colorimetric assays to measure cell proliferation, cytotoxicity, and apoptosis.
ln Vitro |
1. MTT working solution preparation: dissolve MTT in PBS to provide 5 mg/mL MTT. 2. 2.1 Cell seeding (96-well plate): The single cells are prepared and suspended in a culture medium containing 10% FBS. They are then seeded in 96-well plates, with 1,000–10,000 cells per well and a 100 μL volume in each well. 2.2 37°C for culture cells. Incubate for 24-72 hours at 5% CO2. 2.3 Fill each well with 10 μL MTT, then centrifuge the suspended cells after 4 hours of incubation and discarding the supernatant. 2.4 To totally dissolve the crystals, add 100 μL of DMSO and mix for ten minutes. 2.5 To track the rise in absorbance at OD=562 nm, use an absorbance plate reader.
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References |
[1]. Berridge MV, et al. Tetrazolium dyes as tools in cell biology: new insights into their cellular reduction. Biotechnol Annu Rev. 2005;11:127-52.
[2]. Berridge MV, et al. Tetrazolium dyes as tools in cell biology: new insights into their cellular reduction. Biotechnol Annu Rev. 2005;11:127-52. |
Molecular Formula |
C18H16BRN5S
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Molecular Weight |
414.32
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CAS # |
298-93-1
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Appearance |
Typically exists as solids (or liquids in special cases) at room temperature
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SMILES |
CC1=C(C)N=C(N2N=C(C3=CC=CC=C3)N=[N+]2C4=CC=CC=C4)S1.[Br-]
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Synonyms |
Thiazolyl Blue; Thiazolyl Blue Tetrazolium Bromide; Methylthiazolyldiphenyl-tetrazolium bromide
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HS Tariff Code |
2934.99.9001
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Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture and light. |
Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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Solubility (In Vitro) |
DMSO : ~25 mg/mL (~60.34 mM)
H2O : ~0.88 mg/mL (~2.12 mM) |
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Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.08 mg/mL (5.02 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.08 mg/mL (5.02 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C,1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. View More
Solubility in Formulation 3: 6.67 mg/mL (16.10 mM) in PBS (add these co-solvents sequentially from left to right, and one by one), clear solution; with ultrasonication. |
Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
1 mM | 2.4136 mL | 12.0680 mL | 24.1359 mL | |
5 mM | 0.4827 mL | 2.4136 mL | 4.8272 mL | |
10 mM | 0.2414 mL | 1.2068 mL | 2.4136 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration: mg/mL;
Method for preparing DMSO stock solution: mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation::Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O,mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.