| Size | Price | |
|---|---|---|
| 500mg | ||
| 1g | ||
| Other Sizes |
| ln Vitro |
In activated glial cells, MW-150 dihydrochloride dihydrate inhibits in a concentration-dependent manner the ability of endogenous p38αMAPK to phosphorylate the endogenous substrate MK2 [1]. In a concentration-dependent manner, MW-150 dihydrochloride dihydrate inhibits activated glial cells' increased production of IL-1β. MK2 and IL-1β have respective IC50 values of 332 nM and 936 nM[1].
|
|---|---|
| ln Vivo |
APP/PS1 transgenic (Tg) mice's performance in tests is improved by MW-150 dihydrochloride dihydrate (2.5 mg/kg; PO daily for 3–4 months) in the radial arm water maze (RAWM) and contextual fear conditioning[1]. Treatment of APPNLh/NLh × PSP264L/P264L knock-in mice (without amyloid precursor protein overexpression) with MW-150 dihydrochloride dihydrate (2.5 mg/kg; i.p.; once daily for 14 days) resulted in RAWM behavior that was indistinguishable from that of WT mice. 1].
|
| References |
| Molecular Formula |
C24H29CL2N5O2
|
|---|---|
| Molecular Weight |
490.425363302231
|
| Exact Mass |
489.169
|
| CAS # |
1661020-92-3
|
| Related CAS # |
MW-150;1628502-91-9;MW-150 hydrochloride;1923773-01-6
|
| PubChem CID |
131747633
|
| Appearance |
Typically exists as solid at room temperature
|
| Hydrogen Bond Donor Count |
4
|
| Hydrogen Bond Acceptor Count |
7
|
| Rotatable Bond Count |
3
|
| Heavy Atom Count |
33
|
| Complexity |
513
|
| Defined Atom Stereocenter Count |
0
|
| InChi Key |
URBRIDWUCWBFOU-UHFFFAOYSA-N
|
| InChi Code |
InChI=1S/C24H23N5.2ClH.2H2O/c1-28-12-14-29(15-13-28)23-17-22(19-8-10-25-11-9-19)24(27-26-23)21-7-6-18-4-2-3-5-20(18)16-21;;;;/h2-11,16-17H,12-15H2,1H3;2*1H;2*1H2
|
| Chemical Name |
6-(4-methylpiperazin-1-yl)-3-naphthalen-2-yl-4-pyridin-4-ylpyridazine;dihydrate;dihydrochloride
|
| HS Tariff Code |
2934.99.9001
|
| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
DMSO : ~20.83 mg/mL (~42.47 mM)
|
|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.08 mg/mL (4.24 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.08 mg/mL (4.24 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.0390 mL | 10.1951 mL | 20.3903 mL | |
| 5 mM | 0.4078 mL | 2.0390 mL | 4.0781 mL | |
| 10 mM | 0.2039 mL | 1.0195 mL | 2.0390 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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