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NCC-149 (NCC149; NCC 149) is a novel, potent and selective histone deacetylase 8 (HDAC8) inhibitor with an IC50 of 70 nM.
| ln Vitro |
NCC-149 (5 μM; 3 days) dramatically lowers the levels of NeuN expression in P19 cells [1]. In a dose-dependent manner, NCC-149 (0-40 μM; 4 days) decreases the body size of embryoid cells [1]. NCC-149 (5 and 20 μM; 24 hours) inhibits the proliferation of P19 cells [1]. Through G2/M phase arrest, NCC-149 (2.5 and 5 μM; 24 hours) retards the development of cells [1].
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| Cell Assay |
Western Blot Analysis[1]
Cell Types: P19 Cell Tested Concentrations: 5 μM Incubation Duration: 3 days Experimental Results: NeuN expression levels were Dramatically diminished. Cell proliferation assay[1] Cell Types: P19 Cell Tested Concentrations: 5 and 20 μM Incubation Duration: 24 hrs (hours) Experimental Results: Cell proliferation was downregulated. Cell cycle analysis [1] Cell Types: P19 cells Tested Concentrations: 2.5 and 5 μM Incubation Duration: 24 h Experimental Results: It resulted in a significant increase in G2/M phase cells and a slight decrease in S phase cells. RT-PCR[1] Cell Types: P19 Cell Tested Concentrations: 25 μM or 2.5 and 5 μM Incubation Duration: 4 days (25 μM) or 48 hrs (hours) (2.5 and 5 μM) Experimental Results: No reduction in HDAC8 expression at the mRNA level. Dramatically and partially diminished cyclin B1 and cyclin A2 gene expression, respectively. |
| References |
| Molecular Formula |
C16H14N4O2S
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|---|---|
| Molecular Weight |
326.374
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| Exact Mass |
326.083
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| CAS # |
1316652-41-1
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| PubChem CID |
135567459
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| Appearance |
Off-white to light yellow solid powder
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| Density |
1.4±0.1 g/cm3
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| Melting Point |
153 °C(dec.)
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| Index of Refraction |
1.691
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| LogP |
1.6
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| Hydrogen Bond Donor Count |
2
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| Hydrogen Bond Acceptor Count |
5
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| Rotatable Bond Count |
5
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| Heavy Atom Count |
23
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| Complexity |
392
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| Defined Atom Stereocenter Count |
0
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| InChi Key |
DORPIZJGSLWDIY-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C16H14N4O2S/c21-16(18-22)13-6-4-5-12(9-13)15-10-20(19-17-15)11-23-14-7-2-1-3-8-14/h1-10,22H,11H2,(H,18,21)
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| Chemical Name |
N-hydroxy-3-[1-(phenylsulfanylmethyl)triazol-4-yl]benzamide
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| Synonyms |
NCC149 NCC 149 NCC-149
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ~125 mg/mL (~383.00 mM)
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|---|---|
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 3.0640 mL | 15.3200 mL | 30.6401 mL | |
| 5 mM | 0.6128 mL | 3.0640 mL | 6.1280 mL | |
| 10 mM | 0.3064 mL | 1.5320 mL | 3.0640 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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