| Size | Price | Stock | Qty |
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| 5mg |
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| 10mg |
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| 50mg |
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| 100mg |
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| Other Sizes |
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| ln Vitro |
In PC3 cells, NCX4040 dramatically increases the production of hydrogen peroxide, which causes oxidative stress and mitochondrial depolarization. These events result in lipid peroxidation, cell cycle arrest, inhibition of colony expansion, and induction of apoptosis [1]. NCX4040 (10-50 μM) depolymerizes F-actin and induces anoikis, which impairs PC3 cells' ability to migrate [1]. In PC3 cells, NCX4040 suppresses the expression of cyclin D1. Important anti-apoptotic markers, including survivin, X-linked inhibitor of apoptosis (XIAP), inhibitor of apoptosis protein 1 (cIAP1), and Cellular-Myc (c-Myc), were significantly downregulated, according to Western blot analysis[1]. Phosphohistone H2AX (pH2AX), cleaved caspase 3, and cleaved poly[ADP-ribose] polymerase 1 (PARP1) are all upregulated in cells treated with NCX4040- [1].
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| References |
[1]. Somaiah Chinnapaka, et al. Nitro aspirin (NCX4040) induces apoptosis in PC3 metastatic prostate cancer cells via hydrogen peroxide (H 2 O 2)-mediated oxidative stress. Free Radic Biol Med. 2019 Nov 1;143:494-509.
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| Molecular Formula |
C16H13NO7
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|---|---|
| Molecular Weight |
331.27700
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| Exact Mass |
331.069
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| CAS # |
287118-97-2
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| PubChem CID |
9818919
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| Appearance |
Typically exists as solid at room temperature
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| LogP |
3.062
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| Hydrogen Bond Donor Count |
0
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| Hydrogen Bond Acceptor Count |
7
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| Rotatable Bond Count |
7
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| Heavy Atom Count |
24
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| Complexity |
454
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| Defined Atom Stereocenter Count |
0
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| SMILES |
CC(=O)OC1=CC=CC=C1C(=O)OC2=CC=C(C=C2)CO[N+](=O)[O-]
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| InChi Key |
CTHNKWFUDCMLIQ-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C16H13NO7/c1-11(18)23-15-5-3-2-4-14(15)16(19)24-13-8-6-12(7-9-13)10-22-17(20)21/h2-9H,10H2,1H3
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| Chemical Name |
[4-(nitrooxymethyl)phenyl] 2-acetyloxybenzoate
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ~100 mg/mL (~301.86 mM)
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|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (7.55 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 3.0186 mL | 15.0930 mL | 30.1859 mL | |
| 5 mM | 0.6037 mL | 3.0186 mL | 6.0372 mL | |
| 10 mM | 0.3019 mL | 1.5093 mL | 3.0186 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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