| Size | Price | Stock | Qty |
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| 25mg |
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| 50mg |
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| 100mg |
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| 250mg |
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| 500mg |
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NI-57 is a potent inhibitor of the bromodomains of BRPF proteins that binds to BRPF1B, BRPF2, and BRPF3 with Kd values of 31, 108, and 408 nM, respectively
| ln Vitro |
NI-57 is an inhibitor of the plant homeodomain finger domain (BRPF) and the bromodomain with IC50 values of 3.1, 46, and 140 nM for BRPF1, BRPF2 (BRD1), and BRPF3, respectively. NI-57 exhibits a Kd of 31 ± 2 nM for the BRD of BRPF1, 110 ± 13 nM for BRD1, and 410 ± 47 nM for BRPF3. In contrast, the binding to BRD9 is rather poor, with a Kd of 1000 ± 130 nM. Measurements with titration calorimeter. TRIM24 (IC50, 1600 nM), BRD9 (IC50, 520 nM), and BRD4 (BD1) (IC50, 3700 nM) were all targets of little activity for NI-57. With GI50 values of 10.4 μM (NCI-H1703 cells), 14.7 μM (DMS114), 15.6 μM (HRA-19), and 16.6 μM (RERF-LC-Sq1), NI-57 similarly suppresses BRPF BRD in the nucleus but has no effect on the proliferation of numerous cancer cell lines. Furthermore, CCL-22 gene expression was decreased by 27.7 ± 9.4% upon NI-57 (10 μM)-induced BRPF1 inhibition [1].
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| ln Vivo |
In mice, NI-57 exhibits a high oral bioavailability [1].
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| References |
| Molecular Formula |
C19H17N3O4S
|
|---|---|
| Molecular Weight |
383.420983076096
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| Exact Mass |
383.094
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| CAS # |
1883548-89-7
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| Related CAS # |
1883548-89-7;
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| PubChem CID |
91827373
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| Appearance |
White to off-white solid powder
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| LogP |
2.1
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| Hydrogen Bond Donor Count |
1
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| Hydrogen Bond Acceptor Count |
6
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| Rotatable Bond Count |
4
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| Heavy Atom Count |
27
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| Complexity |
764
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| Defined Atom Stereocenter Count |
0
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| SMILES |
C1(S(NC2C=CC3=C(C=2)C=C(C)C(=O)N3C)(=O)=O)=CC=C(C#N)C=C1OC
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| InChi Key |
UEMQPCYDWCSVCU-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C19H17N3O4S/c1-12-8-14-10-15(5-6-16(14)22(2)19(12)23)21-27(24,25)18-7-4-13(11-20)9-17(18)26-3/h4-10,21H,1-3H3
|
| Chemical Name |
4-cyano-N-(1,3-dimethyl-2-oxo-1,2-dihydroquinolin-6-yl)-2-methoxybenzenesulfonamide
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| Synonyms |
NI-57 NI 57 NI57.
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ~100 mg/mL (~260.81 mM)
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|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: 2.5 mg/mL (6.52 mM) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), suspension solution; with sonication.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (6.52 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.6081 mL | 13.0405 mL | 26.0811 mL | |
| 5 mM | 0.5216 mL | 2.6081 mL | 5.2162 mL | |
| 10 mM | 0.2608 mL | 1.3041 mL | 2.6081 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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