NLG919

Alias: IDO-IN 7; IDO IN 7; RG6078 analog; GDC-0919; IDO IN-7;GDC0919 analog; GDC 0919; NLG919; NLG 919 analog; NLG-919; RG-6078; RG 6078.
Cat No.:V0939 Purity: ≥98%
NLG919 analog (RG6078 analog; GDC-0919 analog; GDC0919 analog; IDO-IN-7; NLG-919 analog) is a novel, potent and orally bioavailable inhibitor of IDO (indoleamine-(2,3)-dioxygenase) pathway with potential immunomodulating and antitumor activity.
NLG919 Chemical Structure CAS No.: 1402836-58-1
Product category: IDO
This product is for research use only, not for human use. We do not sell to patients.
Size Price Stock Qty
5mg
10mg
25mg
50mg
100mg
250mg
500mg
Other Sizes
Official Supplier of:
Alternate Text
Alternate Text
Alternate Text
Alternate Text
Alternate Text
Alternate Text
Alternate Text
Alternate Text
Alternate Text
Alternate Text
Alternate Text
Alternate Text
Alternate Text
Alternate Text
Alternate Text
Alternate Text
Alternate Text
Alternate Text
Alternate Text
Alternate Text
Alternate Text
Alternate Text
Alternate Text
Alternate Text
Alternate Text
Alternate Text
Alternate Text
Alternate Text
Alternate Text
Alternate Text
Alternate Text
Alternate Text

 

  • Business Relationship with 5000+ Clients Globally
  • Major Universities, Research Institutions, Biotech & Pharma
  • Citations by Top Journals: Nature, Cell, Science, etc.
Top Publications Citing lnvivochem Products
Purity & Quality Control Documentation

Purity: ≥98%

Purity: ≥98%

Purity: ≥98%

Product Description

NLG919 analog (RG6078 analog; GDC-0919 analog; GDC0919 analog; IDO-IN-7; NLG-919 analog) is a novel, potent and orally bioavailable inhibitor of IDO (indoleamine-(2,3)-dioxygenase) pathway with potential immunomodulating and antitumor activity. It inhibits IDO1 with Ki/EC50 of 7 nM/75 nM in cell-free assays. IDO1 catalyzes the conversion of tryptophan into kynurenine. By inhibiting IDO1 and decreasing kynurenine in tumor cells, NLG919 analog increases tryptophan levels, restores the proliferation and activation of immune cells (e.g. NK cells, T-lymphocytes), leading to a reduction in tumor-associated regulatory T-cells.

Biological Activity I Assay Protocols (From Reference)
ln Vitro
Strong IDO1 inhibitor IDO-IN-7 (analogue of NLG-919) has an IC50 of 38 nM. IDO-IN-7's binding mode to IDO1 is accessible through experimentation and demonstrates a direct coordination interaction with the ferric heme's sixth coordination site. In previous research, IDO-IN-7 was utilized as a reference drug to build immunostimulatory nanomicellar carriers and verify high-throughput screening assays for IDO1 inhibition[1].
ln Vivo
In mice, a single oral administration of NLG919 reduces the concentration of plasma and tissue Kyn by ~ 50%. In mice bearing B16F10 tumors, NLG919 markedly enhances the antitumor responses of naive, resting pmel-1 cells to vaccination with cognate hgp100 peptide plus CpG-1826 in IFA.
Animal Protocol
Dissolved in water at 3 mg/mL; 6 mg/day injected via IP, or administered subcutaneously at 1 mg/dose twice a day via injection plus 360 μg/day via an SC osmotic pump.
Mice bearing large established B16F10 tumor
References
[1]. Coletti A, et al. Fragment-based approach to identify IDO1 inhibitor building blocks. Eur J Med Chem. 2017 Dec 1;141:169-177
These protocols are for reference only. InvivoChem does not independently validate these methods.
Physicochemical Properties
Molecular Formula
C18H22N2O
Molecular Weight
282.38
CAS #
1402836-58-1
SMILES
OC(C1CCCCC1)CC(C2=C3C=CC=C2)N4C3=CN=C4
Synonyms
IDO-IN 7; IDO IN 7; RG6078 analog; GDC-0919; IDO IN-7;GDC0919 analog; GDC 0919; NLG919; NLG 919 analog; NLG-919; RG-6078; RG 6078.
Storage

Powder      -20°C    3 years

                     4°C     2 years

In solvent   -80°C    6 months

                  -20°C    1 month

Shipping Condition
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
Solubility Data
Solubility (In Vitro)
DMSO: 15 mg/mL warmed (53.1 mM)
Water:<1 mg/mL
Ethanol: 30 mg/mL (106.2 mM)
Preparing Stock Solutions 1 mg 5 mg 10 mg
1 mM 3.5413 mL 17.7066 mL 35.4133 mL
5 mM 0.7083 mL 3.5413 mL 7.0827 mL
10 mM 0.3541 mL 1.7707 mL 3.5413 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.

Calculator

Molarity Calculator allows you to calculate the mass, volume, and/or concentration required for a solution, as detailed below:

  • Calculate the Mass of a compound required to prepare a solution of known volume and concentration
  • Calculate the Volume of solution required to dissolve a compound of known mass to a desired concentration
  • Calculate the Concentration of a solution resulting from a known mass of compound in a specific volume
An example of molarity calculation using the molarity calculator is shown below:
What is the mass of compound required to make a 10 mM stock solution in 5 ml of DMSO given that the molecular weight of the compound is 350.26 g/mol?
  • Enter 350.26 in the Molecular Weight (MW) box
  • Enter 10 in the Concentration box and choose the correct unit (mM)
  • Enter 5 in the Volume box and choose the correct unit (mL)
  • Click the “Calculate” button
  • The answer of 17.513 mg appears in the Mass box. In a similar way, you may calculate the volume and concentration.

Dilution Calculator allows you to calculate how to dilute a stock solution of known concentrations. For example, you may Enter C1, C2 & V2 to calculate V1, as detailed below:

What volume of a given 10 mM stock solution is required to make 25 ml of a 25 μM solution?
Using the equation C1V1 = C2V2, where C1=10 mM, C2=25 μM, V2=25 ml and V1 is the unknown:
  • Enter 10 into the Concentration (Start) box and choose the correct unit (mM)
  • Enter 25 into the Concentration (End) box and select the correct unit (mM)
  • Enter 25 into the Volume (End) box and choose the correct unit (mL)
  • Click the “Calculate” button
  • The answer of 62.5 μL (0.1 ml) appears in the Volume (Start) box
g/mol

Molecular Weight Calculator allows you to calculate the molar mass and elemental composition of a compound, as detailed below:

Note: Chemical formula is case sensitive: C12H18N3O4  c12h18n3o4
Instructions to calculate molar mass (molecular weight) of a chemical compound:
  • To calculate molar mass of a chemical compound, please enter the chemical/molecular formula and click the “Calculate’ button.
Definitions of molecular mass, molecular weight, molar mass and molar weight:
  • Molecular mass (or molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
  • Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.
/

Reconstitution Calculator allows you to calculate the volume of solvent required to reconstitute your vial.

  • Enter the mass of the reagent and the desired reconstitution concentration as well as the correct units
  • Click the “Calculate” button
  • The answer appears in the Volume (to add to vial) box
In vivo Formulation Calculator (Clear solution)
Step 1: Enter information below (Recommended: An additional animal to make allowance for loss during the experiment)
Step 2: Enter in vivo formulation (This is only a calculator, not the exact formulation for a specific product. Please contact us first if there is no in vivo formulation in the solubility section.)
+
+
+

Calculation results

Working concentration mg/mL;

Method for preparing DMSO stock solution mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.

Method for preparing in vivo formulation:Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O,mix and clarify.

(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
             (2) Be sure to add the solvent(s) in order.

Biological Data
  • NLG919
    IDO-blockade synergizes with chemo-radiation therapy.J Immunother Cancer.2014Jul 7;2:21.
  • NLG919

    Inhibition or absence of IDO triggers widespread complement deposition in tumors after chemo-radiation therapy.J Immunother Cancer.2014Jul 7;2:21.
  • NLG919

    Complement is mechanistically required for IDO-blockade to synergize with chemo-radiation therapy.J Immunother Cancer.2014Jul 7;2:21.
  • NLG919

    Bone marrow-derived leukocytes aggregate around tumor blood vessels after chemotherapy treatment alone.J Immunother Cancer.2014Jul 7;2:21.
  • NLG919

    IDO-blockade drives vascular activation after chemotherapy and tumor necrosis after chemo-radiation therapy.J Immunother Cancer.2014Jul 7;2:21.
  • NLG919

    Proposed sequence of events.J Immunother Cancer.2014Jul 7;2:21.
Contact Us