Size | Price | Stock | Qty |
---|---|---|---|
10mg |
|
||
25mg |
|
||
50mg |
|
||
100mg |
|
||
250mg |
|
||
Other Sizes |
|
Purity: ≥98%
NSC 144303 (also known as Ubiquitin Isopeptidase Inhibitor I, G5) is a novel and potent apoptosome-independent caspase and apoptosis activator with IC50 values of 1.76 and 1.6 μM in E1A and E1A/C9DN cells, respectively. Additionally, it is a strong inhibitor of DUB activity in general, which causes acute proteotoxic stress and apoptosis and causes apoptosis in Bcl-2 over-expressing cells.
Targets |
E1A cells (IC50 = 1.76 μM); E1A/C9DN cells (IC50 = 1.6 μM)
|
---|---|
ln Vitro |
By blocking ubiquitin peptidase, it suppresses the ubiquitin kinase body system. Kinase-independent pathways can be activated by G5. G5 has the ability to trigger several unusual kinase pathways, such as the apoptotic body's Bcl-2-dependent mitochondrial pathway. It can also increase the stability of the BH3 protein Noxa, the antagonist Smac, and the death receptor pathway. With IC50s of 1.76 and 1.6 μM in E1A and E1A/C9DN cells, respectively, G5 is a powerful inducer of cell death [1].
|
References |
[1]. Aleo E, et al. Identification of new compounds that trigger apoptosome-independent caspase activation and apoptosis. Cancer Res. 2006 Sep 15;66(18):9235-44
|
Molecular Formula |
C19H14N2O7S
|
---|---|
Molecular Weight |
414.388663768768
|
Exact Mass |
414.0522
|
Elemental Analysis |
C, 55.07; H, 3.41; N, 6.76; O, 27.03; S, 7.74
|
CAS # |
108477-18-5
|
Related CAS # |
108477-18-5
|
Appearance |
Solid powder
|
SMILES |
C\1S(=O)(=O)C/C(=C\C2=CC=C(C=C2)[N+](=O)[O-])/C(=O)/C1=C/C3=CC=C(C=C3)[N+](=O)[O-]
|
InChi Key |
SSAMIOWVUGBIJK-KAVGSWPWSA-N
|
InChi Code |
InChI=1S/C19H14N2O7S/c22-19-15(9-13-1-5-17(6-2-13)20(23)24)11-29(27,28)12-16(19)10-14-3-7-18(8-4-14)21(25)26/h1-10H,11-12H2/b15-9+,16-10+
|
Chemical Name |
(3Z,5Z)-3,5-bis[(4-nitrophenyl)methylidene]-1,1-dioxothian-4-one
|
Synonyms |
Ubiquitin Isopeptidase Inhibitor I; NSC-144303; NSC144303; NSC 144303; G5
|
HS Tariff Code |
2934.99.9001
|
Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product is not stable in solution, please use freshly prepared working solution for optimal results. |
Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
Solubility (In Vitro) |
DMSO: ~50 mg/mL (~120.7 mM; Need ultrasonic)
THF: ~3.4 mg/mL (~8.3 mM; Need ultrasonic) Acetone: ~2.9 mg/mL (~6.9 mM; Need ultrasonic) Ethanol: < 1 mg/mL (ultrasonic) (insoluble) |
---|---|
Solubility (In Vivo) |
Solubility in Formulation 1: 10 mg/mL (24.13 mM) in 15% Cremophor EL 85% Saline (add these co-solvents sequentially from left to right, and one by one), suspension solution; with sonication.
Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution.  (Please use freshly prepared in vivo formulations for optimal results.) |
Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
1 mM | 2.4132 mL | 12.0659 mL | 24.1319 mL | |
5 mM | 0.4826 mL | 2.4132 mL | 4.8264 mL | |
10 mM | 0.2413 mL | 1.2066 mL | 2.4132 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration: mg/mL;
Method for preparing DMSO stock solution: mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation::Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O,mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.