| Size | Price | Stock | Qty |
|---|---|---|---|
| 5mg |
|
||
| 10mg |
|
||
| 25mg |
|
||
| 50mg |
|
||
| 100mg |
|
||
| 250mg |
|
||
| Other Sizes |
|
Purity: ≥98%
NSC59984 is a novel and potenl p53 activator with potential anticancer activity. It works by causing the degradation of mutant p53 protein and the activation of p73.
| Targets |
p53
|
|||
|---|---|---|---|---|
| ln Vitro |
NSC59984 specifically induces p73-dependent cell death and restores p53 pathway signaling in mutant p53-expressing human colorectal cancer cells. Additionally, NSC59984 causes cell death in a panel of cancer cell lines with various p53 mutations while causing little to no genotoxicity in healthy cells. [1]
|
|||
| ln Vivo |
NSC59984 (45 mg/kg, i.p.) significantly inhibits tumor growth in nude mice with DLD-1 xenograft tumors in a p73-dependent manner. [1]
|
|||
| Cell Assay |
Cells are seeded at 5,000 cells per well on 96-well plates. The manufacturer's recommended procedure is followed when mixing cells with an equal volume of CellTiter-Glo reagents, and the IVIS imager is used to measure the bioluminescence.
|
|||
| Animal Protocol |
|
|||
| References |
|
| Molecular Formula |
C12H15N3O4
|
|
|---|---|---|
| Molecular Weight |
265.27
|
|
| Exact Mass |
265.106
|
|
| Elemental Analysis |
C, 54.33; H, 5.70; N, 15.84; O, 24.12
|
|
| CAS # |
803647-40-7
|
|
| Related CAS # |
|
|
| PubChem CID |
5356520
|
|
| Appearance |
Light yellow to yellow solid powder
|
|
| Density |
1.3±0.1 g/cm3
|
|
| Boiling Point |
451.8±45.0 °C at 760 mmHg
|
|
| Flash Point |
227.1±28.7 °C
|
|
| Vapour Pressure |
0.0±1.1 mmHg at 25°C
|
|
| Index of Refraction |
1.596
|
|
| LogP |
0.82
|
|
| Hydrogen Bond Donor Count |
0
|
|
| Hydrogen Bond Acceptor Count |
5
|
|
| Rotatable Bond Count |
2
|
|
| Heavy Atom Count |
19
|
|
| Complexity |
372
|
|
| Defined Atom Stereocenter Count |
0
|
|
| SMILES |
O=C(N1CCN(C)CC1)C=CC1=CC=C([N+](=O)[O-])O1
|
|
| InChi Key |
QKTRIGNWBRHBFV-DUXPYHPUSA-N
|
|
| InChi Code |
InChI=1S/C12H15N3O4/c1-13-6-8-14(9-7-13)11(16)4-2-10-3-5-12(19-10)15(17)18/h2-5H,6-9H2,1H3/b4-2+
|
|
| Chemical Name |
(E)-1-(4-methylpiperazin-1-yl)-3-(5-nitrofuran-2-yl)prop-2-en-1-one
|
|
| Synonyms |
NSC59984; NSC 59984; NSC-59984
|
|
| HS Tariff Code |
2934.99.9001
|
|
| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
|
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
|
|||
|---|---|---|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (9.42 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (9.42 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 3.7697 mL | 18.8487 mL | 37.6974 mL | |
| 5 mM | 0.7539 mL | 3.7697 mL | 7.5395 mL | |
| 10 mM | 0.3770 mL | 1.8849 mL | 3.7697 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
|
|---|
|
|
Products are for research use only; We do not sell to patients
Copyright 2020 InvivoChem LLC | All Rights Reserved
COA
