Nucleozin

Cat No.:V3383 Purity: ≥98%
Nucleozin is a novel potent antivirus agent that targets influenza A nucleoprotein (NP), which is a multifunctional, RNA-binding protein necessary for virus replication.
Nucleozin Chemical Structure CAS No.: 341001-38-5
Product category: Influenza Virus
This product is for research use only, not for human use. We do not sell to patients.
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Purity & Quality Control Documentation

Purity: ≥98%

Product Description

Nucleozin is a novel potent antivirus agent that targets influenza A nucleoprotein (NP), which is a multifunctional, RNA-binding protein necessary for virus replication. Nucleozin is effective in fighting H1N1, H3N2, and H5N1 flu virus strains by inducing the formation of NP aggregates and antagonizing its nuclear accumulation, leading to cessation of viral replication. As influenza viruses have developed resistance towards current drugs, new inhibitors that prevent viral replication through different inhibitory mechanisms (e.g. targeting the influenza A nucleoprotein) are useful.

Biological Activity I Assay Protocols (From Reference)
ln Vitro
Nucleozin impedes the infection of influenza A/WSN/33, H3N2, and Vietnam/1194/04 (H5N1) in MDCK cells, with EC50 values of 0.069 μM, 0.16 μM, and 0.33 μM in the plaque reduction assay (PRA), correspondingly. In multicycle growth assays, nucleozin completely inhibits virus production at 1 μM and severely suppresses viral growth at 0.1 μM.
ln Vivo
Mice exposed to fatal doses of avian influenza A H5N1 are shielded by nucleozin[1].
References

[1]. Nat Biotechnol. 2010;28(6):600-605.

[2]. Proc Natl Acad Sci U S A.2010 Nov 9;107(45):19151-6.

[3]. Anal Chem.2012 Aug 7;84(15):6391-9.

These protocols are for reference only. InvivoChem does not independently validate these methods.
Physicochemical Properties
Molecular Formula
C21H19N4O4CL
Molecular Weight
426.85296
Exact Mass
426.11
Elemental Analysis
C, 59.09; H, 4.49; Cl, 8.30; N, 13.13; O, 14.99
CAS #
341001-38-5
Appearance
Solid powder
SMILES
O=C(N1CCN(C2=CC=C([N+]([O-])=O)C=C2Cl)CC1)C3=C(C)ON=C3C4=CC=CC=C4
InChi Key
OWXBJAPOSQSWAO-UHFFFAOYSA-N
InChi Code
InChI=1S/C21H19ClN4O4/c1-14-19(20(23-30-14)15-5-3-2-4-6-15)21(27)25-11-9-24(10-12-25)18-8-7-16(26(28)29)13-17(18)22/h2-8,13H,9-12H2,1H3
Chemical Name
1-(2-Chloro-4-nitrophenyl)-4-[(5-methyl-3-phenyl-4-isoxazolyl)carbonyl]-piperazine
HS Tariff Code
2934.99.9001
Storage

Powder      -20°C    3 years

                     4°C     2 years

In solvent   -80°C    6 months

                  -20°C    1 month

Shipping Condition
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
Solubility Data
Solubility (In Vitro)
DMSO : 15~20 mg/mL ( 35.14~46.85 mM)
Solubility (In Vivo)
10% DMSO+40% PEG300+5% Tween-80+45% Saline: ≥ 2 mg/mL (4.69 mM) (Please use freshly prepared in vivo formulations for optimal results.)
Preparing Stock Solutions 1 mg 5 mg 10 mg
1 mM 2.3427 mL 11.7137 mL 23.4274 mL
5 mM 0.4685 mL 2.3427 mL 4.6855 mL
10 mM 0.2343 mL 1.1714 mL 2.3427 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.

Calculator

Molarity Calculator allows you to calculate the mass, volume, and/or concentration required for a solution, as detailed below:

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An example of molarity calculation using the molarity calculator is shown below:
What is the mass of compound required to make a 10 mM stock solution in 5 ml of DMSO given that the molecular weight of the compound is 350.26 g/mol?
  • Enter 350.26 in the Molecular Weight (MW) box
  • Enter 10 in the Concentration box and choose the correct unit (mM)
  • Enter 5 in the Volume box and choose the correct unit (mL)
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  • The answer of 17.513 mg appears in the Mass box. In a similar way, you may calculate the volume and concentration.

Dilution Calculator allows you to calculate how to dilute a stock solution of known concentrations. For example, you may Enter C1, C2 & V2 to calculate V1, as detailed below:

What volume of a given 10 mM stock solution is required to make 25 ml of a 25 μM solution?
Using the equation C1V1 = C2V2, where C1=10 mM, C2=25 μM, V2=25 ml and V1 is the unknown:
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  • Enter 25 into the Concentration (End) box and select the correct unit (mM)
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  • The answer of 62.5 μL (0.1 ml) appears in the Volume (Start) box
g/mol

Molecular Weight Calculator allows you to calculate the molar mass and elemental composition of a compound, as detailed below:

Note: Chemical formula is case sensitive: C12H18N3O4  c12h18n3o4
Instructions to calculate molar mass (molecular weight) of a chemical compound:
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Definitions of molecular mass, molecular weight, molar mass and molar weight:
  • Molecular mass (or molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
  • Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.
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Reconstitution Calculator allows you to calculate the volume of solvent required to reconstitute your vial.

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In vivo Formulation Calculator (Clear solution)
Step 1: Enter information below (Recommended: An additional animal to make allowance for loss during the experiment)
Step 2: Enter in vivo formulation (This is only a calculator, not the exact formulation for a specific product. Please contact us first if there is no in vivo formulation in the solubility section.)
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Calculation results

Working concentration mg/mL;

Method for preparing DMSO stock solution mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.

Method for preparing in vivo formulation:Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O,mix and clarify.

(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
             (2) Be sure to add the solvent(s) in order.

Biological Data
  • Chemical structures and biological activities of nucleozin and related compounds. [1].Nat Biotechnol. 2010;28(6):600-605.
  • Influenza A NP is the molecular target of nucleozin. [1].Nat Biotechnol. 2010;28(6):600-605.
  • Nucleozin interacts with NP and causes NP aggregation. [1].Nat Biotechnol. 2010;28(6):600-605.
  • Efficacies of nucleozin in a mice H5N1 virus infection model. [1].Nat Biotechnol. 2010;28(6):600-605.
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