Size | Price | Stock | Qty |
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5mg |
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10mg |
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25mg |
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50mg |
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100mg |
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Other Sizes |
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Targets |
PTEN; COX-2; PI3K; Akt
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ln Vitro |
Oroxin B (0-2 μM, 48 hours) causes the human TRAY cell line (SMMC 7721) to glow during proliferation (48 hours) and induce cell filling (12 hours) [1]. In SMMC 7721, oroxin B (0-2 μM, 48) increases PTEN and suppresses p-AKT, COX-2, VEGF, and PI3K[1]. In Raji cells, Oroxin B (160 μM, 24 hours) reduced IL-1β-induced inflammation-related indicators (iNOS, COX-2, TNF-α, IL-6: rhodamine-labeled glyburin arteries) and torsion-induced ER stress hormones (dorsal markers)[2].
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ln Vivo |
In the human simulated cell (Raji Cell) xenograft model, oroxin B (30 mg/kg, intraperitoneal injection, 28 days) can stimulate the middle part of the ER in simulated cells and prevent tumor growth [2]. When knee OA is caused by medial meniscus (DMM), oroxin B (160 μM, 10 μL) injection into the probing knee joint can preserve the unstable articular cartilage [3]. For HFD feeding, Oroxin B (200 mg/kg/day, side gavage) may be utilized.
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Cell Assay |
RT-PCR[2]
Cell Types: Raji cells Tested Concentrations: 0-40 μM Incubation Duration: 48 h Experimental Results: ER stress diminished mRNA levels of major genes (GRP78 and ATF6. and IL-1β) [3]. |
Animal Protocol |
Animal/Disease Models: HFD-fed rats [4]
Doses: 200 mg/kg/day Route of Administration: po (oral gavage) Experimental Results: diminished plasma lipids, LPS, IL-6 and TNF-α levels. Inhibits liver fibrosis by reducing collagen deposition. |
References |
[1]. Li NN, et al. Evidence for the Involvement of COX-2/VEGF and PTEN/Pl3K/AKT Pathway the Mechanism of Oroxin B Treated Liver Cancer. Pharmacogn Mag. 2018 Apr-Jun;14(54):207-213.
[2]. Yang P, et al. Oroxin B selectively induces tumor-suppressive ER stress and concurrently inhibits tumor-adaptive ER stress in B-lymphoma cells for effective anti-lymphoma therapy. Toxicol Appl Pharmacol. 2015 Oct 15;288(2):269-79. [3]. Lu R, et al. Oroxin B alleviates osteoarthritis through anti-inflammation and inhibition of PI3K/AKT/mTOR signaling pathway and enhancement of autophagy. Front Endocrinol (Lausanne). 2022 Dec 1;13:1060721. [4]. Huang Y, et al. Oroxin B improves metabolic-associated fatty liver disease by alleviating gut microbiota dysbiosis in a high-fat diet-induced rat model. Eur J Pharmacol. 2023 Jul 15;951:175788. |
Molecular Formula |
C27H30O15
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Molecular Weight |
594.5181
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Exact Mass |
594.15847025
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CAS # |
114482-86-9
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Related CAS # |
114482-86-9
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Appearance |
Solid
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SMILES |
C1=CC=C(C=C1)C2=CC(=O)C3=C(C(=C(C=C3O2)O[C@H]4[C@@H]([C@H]([C@@H]([C@H](O4)CO[C@H]5[C@@H]([C@H]([C@@H]([C@H](O5)CO)O)O)O)O)O)O)O)O
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InChi Key |
HAYLVXFWJCKKDW-IJTBWITGSA-N
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InChi Code |
InChI=1S/C27H30O15/c28-8-15-19(31)22(34)24(36)26(41-15)38-9-16-20(32)23(35)25(37)27(42-16)40-14-7-13-17(21(33)18(14)30)11(29)6-12(39-13)10-4-2-1-3-5-10/h1-7,15-16,19-20,22-28,30-37H,8-9H2/t15-,16-,19-,20-,22+,23+,24-,25-,26-,27-/m1/s1
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Chemical Name |
5,6-dihydroxy-2-phenyl-7-[(2S,3R,4S,5S,6R)-3,4,5-trihydroxy-6-[[(2R,3R,4S,5S,6R)-3,4,5-trihydroxy-6-(hydroxymethyl)oxan-2-yl]oxymethyl]oxan-2-yl]oxychromen-4-one
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Synonyms |
Oroxin B
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HS Tariff Code |
2934.99.9001
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Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage. |
Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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Solubility (In Vitro) |
DMSO: 100 mg/mL (~168.2 mM)
H2O: <0.1 mg/mL |
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Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (4.21 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (4.21 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C,1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution.  (Please use freshly prepared in vivo formulations for optimal results.) |
Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
1 mM | 1.6820 mL | 8.4101 mL | 16.8203 mL | |
5 mM | 0.3364 mL | 1.6820 mL | 3.3641 mL | |
10 mM | 0.1682 mL | 0.8410 mL | 1.6820 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration: mg/mL;
Method for preparing DMSO stock solution: mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation::Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O,mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.