Size | Price | Stock | Qty |
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1mg |
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5mg |
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Other Sizes |
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Purity: ≥98%
OSW-1 (Orsaponin) is a naturally occuring and broad spectrum inhibitor of enterovirus replication isolated from Ornithogalum caudatum, it is a specific antagonist of osterol-binding protein (OSBP) and OSBP-related protein 4 (ORP4) with GI50s in the nanomolar range in human cancer lines.
ln Vitro |
OSW-1 has an anti-proliferative effect on sandwich circles and is less cytotoxic to normal cells. The antiproliferative activity of OSW-1 on SW480 and LoVo sandwich circles, as evidenced by cell viability determined by the CCK8 assay, placed the results in comparison with other clinical anticancer treatments. SW480 and LoVo cell lines are obtained from Dukes stage B non-lymphoma and lymphoma metastatic nodules and represent metastatic and metastatic malignancy, respectively. OSW-1 not only demonstrates extraordinarily powerful anti-cancer action in SW480 and LoVo cell lines, with IC50 at nanomolar doses, and is 10-100 times more effective than other anti-cancer medicines, it is cytotoxic to normal epithelial cells The effect is minimal. These data demonstrate that OSW-1 has a high anti-cancer effect, but has a low cytotoxic effect on normal cells [2].
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ln Vivo |
In a nude mouse model implanted with LoVo cells and treated with OSW-1 when tumors became operable, we investigated whether OSW-1 was as effective in vivo. The tumor sizes in the treated mice were, on average, less than those in the control group, according to the results. Significant changes were seen 15–17 days after treatment, and as time went on, the discrepancies grew larger until the animals were killed by the solution. The OSW-1 treated group's tumor weight was considerably lower (P<0.05) than that of the control group. Furthermore, tumors treated with OSW-1 did not show any discernible response. According to these findings, OSW-1 tumors have a noticeable in vivo response and a potent inhibitory effect on blood vessel formation [2].
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References | |
Additional Infomation |
[(2S,3R,4S,5R)-2-[(2S,3R,4S,5S)-3-acetyloxy-2-[[(3S,8R,9S,10R,13S,14S,16S,17S)-3,17-dihydroxy-10,13-dimethyl-17-[(2S)-6-methyl-3-oxoheptan-2-yl]-1,2,3,4,7,8,9,11,12,14,15,16-dodecahydrocyclopenta[a]phenanthren-16-yl]oxy]-5-hydroxyoxan-4-yl]oxy-4,5-dihydroxyoxan-3-yl] 4-methoxybenzoate has been reported in Ornithogalum saundersiae with data available.
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Molecular Formula |
C47H68O15
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Molecular Weight |
873.03
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Exact Mass |
872.456
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CAS # |
145075-81-6
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PubChem CID |
9854230
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Appearance |
White to off-white solid powder
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Density |
1.31 g/cm3
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Boiling Point |
932.5ºC at 760 mmHg
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Flash Point |
267ºC
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Vapour Pressure |
0mmHg at 25°C
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Index of Refraction |
1.592
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LogP |
4.024
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Hydrogen Bond Donor Count |
5
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Hydrogen Bond Acceptor Count |
15
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Rotatable Bond Count |
15
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Heavy Atom Count |
62
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Complexity |
1630
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Defined Atom Stereocenter Count |
17
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SMILES |
[C@H]1(CC2=CC[C@]3([H])[C@@]([H])([C@]2(CC1)C)CC[C@@]1([C@](O)([C@H](C[C@]13[H])O[C@@]1([H])[C@@H]([C@@H](O[C@@]2([H])[C@@H]([C@H]([C@@H](CO2)O)O)OC(C2C=CC(OC)=CC=2)=O)[C@H](CO1)O)OC(=O)C)[C@H](C)C(=O)CCC(C)C)C)O
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InChi Key |
MPXTYZZFIJTPPA-MKQTXCTDSA-N
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InChi Code |
InChI=1S/C47H68O15/c1-24(2)8-15-34(50)25(3)47(55)37(21-33-31-14-11-28-20-29(49)16-18-45(28,5)32(31)17-19-46(33,47)6)60-44-41(59-26(4)48)39(36(52)23-58-44)62-43-40(38(53)35(51)22-57-43)61-42(54)27-9-12-30(56-7)13-10-27/h9-13,24-25,29,31-33,35-41,43-44,49,51-53,55H,8,14-23H2,1-7H3/t25-,29+,31-,32+,33+,35-,36+,37+,38+,39+,40-,41-,43+,44+,45+,46+,47-/m1/s1
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Chemical Name |
(2S,3R,4S,5R)-2-(((2S,3R,4S,5S)-3-acetoxy-2-(((3S,8R,9S,10R,13S,14S,16S,17S)-3,17-dihydroxy-10,13-dimethyl-17-((S)-6-methyl-3-oxoheptan-2-yl)-2,3,4,7,8,9,10,11,12,13,14,15,16,17-tetradecahydro-1H-cyclopenta[a]phenanthren-16-yl)oxy)-5-hydroxytetrahydro-2H-pyran-4-yl)oxy)-4,5-dihydroxytetrahydro-2H-pyran-3-yl
4-methoxybenzoate
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Synonyms |
Orsaponin
OSW-1 OSW1OSW 1
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HS Tariff Code |
2934.99.9001
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Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: (1). This product requires protection from light (avoid light exposure) during transportation and storage. (2). Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture. |
Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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Solubility (In Vitro) |
DMSO : ~100 mg/mL (~114.54 mM)
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Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (2.86 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (2.86 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C,1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. View More
Solubility in Formulation 3: ≥ 2.5 mg/mL (2.86 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. |
Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
1 mM | 1.1454 mL | 5.7272 mL | 11.4544 mL | |
5 mM | 0.2291 mL | 1.1454 mL | 2.2909 mL | |
10 mM | 0.1145 mL | 0.5727 mL | 1.1454 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration: mg/mL;
Method for preparing DMSO stock solution: mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation::Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O,mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.