| Size | Price | Stock | Qty |
|---|---|---|---|
| 500mg |
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| 5g |
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| Other Sizes |
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| ln Vitro |
In a 24-hour period, oxybenzone (benzophenone 3) (25 μM) increased relative RXRα protein levels by 49% and decreased relative RXRβ and RXRγ protein levels by 61% and 56%, respectively [3]. At 7 DIV, caspase-3 levels are increased in initial cultures of mouse neocortical cells by oxybenzone (25-100 μM; 24 hours). RXRα signaling is activated and RXRβ/RXRγ signaling is impaired in oxybenzone-induced apoptosis. In mouse embryonic neural cells, oxybenzone (25 μM; 24 hours) decreases HDAC and HAT activity and prevents global DNA methylation [3].
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|---|---|
| Cell Assay |
Western blot analysis [3]
Cell Types: 7 DIV mouse neocortical cells Tested Concentrations: 25 μM Incubation Duration: 24 hrs (hours) Experimental Results: Exposure to oxybenzone (25 μM) for 24 hrs (hours) diminished relative RXRβ and RXRγ protein levels by 61% respectively and 56%, respectively. OxyBenzone (25 μM) treatment increased relative RXRα protein levels by 49%. |
| References |
[1]. Wnuk A, et al. Prenatal exposure to benzophenone-3 (BP-3) induces apoptosis, disrupts estrogen receptor expression and alters the epigenetic status of mouse neurons. J Steroid Biochem Mol Biol. 2018;182:106-118.
[2]. DiNardo JC, et al. Can oxybenzone cause Hirschsprung's disease?. Reprod Toxicol. 2019;86:98-100. [3]. Wnuk A, et al. Benzophenone-3 Impairs Autophagy, Alters Epigenetic Status, and Disrupts Retinoid X Receptor Signaling in Apoptotic Neuronal Cells. Mol Neurobiol. 2018;55(6):5059-5074. |
| Molecular Formula |
C14H12O3
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|---|---|
| Molecular Weight |
228.2433
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| CAS # |
131-57-7
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| Related CAS # |
Oxybenzone-d5;1219798-54-5
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| Appearance |
Typically exists as solids (or liquids in special cases) at room temperature
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| SMILES |
O(C([H])([H])[H])C1C([H])=C([H])C(=C(C=1[H])O[H])C(C1C([H])=C([H])C([H])=C([H])C=1[H])=O
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ~100 mg/mL (~438.14 mM)
H2O : ~1 mg/mL (~4.38 mM) |
|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (10.95 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (10.95 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. View More
Solubility in Formulation 3: ≥ 2.5 mg/mL (10.95 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. Solubility in Formulation 4: 5 mg/mL (21.91 mM) in PBS (add these co-solvents sequentially from left to right, and one by one), clear solution; with ultrasonication (<60°C). |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 4.3814 mL | 21.9068 mL | 43.8135 mL | |
| 5 mM | 0.8763 mL | 4.3814 mL | 8.7627 mL | |
| 10 mM | 0.4381 mL | 2.1907 mL | 4.3814 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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