| Size | Price | Stock | Qty |
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| 5mg |
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| 10mg |
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| 50mg |
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| Other Sizes |
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| ln Vitro |
In Hep3B cells, orphenibuta can improve methotrexate's (MTX) anti-cancer efficacy [1]. Hep3B cells may be cytotoxically affected by co-treatment with oxyphenbutazone (2.5–7.5 µM; 48 hours) and MTX (0.25–1.0 µM) [1]. Liver cells can be repaired by orphenibuta [1].
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|---|---|
| ln Vivo |
Combining oxyphenbutazone (70 mg/kg/week; oral; divided into two doses; for 13 weeks) with MTX (5.0 or 2.5 mg/kg/week; intraperitoneally) may have anticancer effects [1].
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| Cell Assay |
Cytotoxicity Assay[1]
Cell Types: Hep3B Cell Tested Concentrations: 2.5 µM, 5 µM, 7.5 µM Incubation Duration: 48 hrs (hours) Experimental Results: Enhanced cytotoxicity of MTX. |
| Animal Protocol |
Animal/Disease Models: Wistar strain albino male rats (5-6 weeks; 150-220 g) [1]
Doses: 70 mg/kg/week (co-treatment with MTX 5.0 or 2.5 mg/kg/week) Route of Administration: Oral ; once a week; taken in two divided doses; for 13 weeks. Experimental Results: When combined with MTX, it has potential anti-cancer activity in rats. |
| References |
[1]. Saleem S, et al. Oxyphenbutazone promotes cytotoxicity in rats and Hep3B cellsvia suppression of PGE2 and deactivation of Wnt/β-catenin signaling pathway. Mol Cell Biochem. 2018 Jul;444(1-2):187-196.
[2]. Gold B, et al. Nonsteroidal anti-inflammatory drug sensitizes Mycobacterium tuberculosis to endogenous and exogenous antimicrobials. Proc Natl Acad Sci U S A. 2012 Oct 2;109(40):16004-11. |
| Molecular Formula |
C19H20N2O3
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|---|---|
| Molecular Weight |
324.38
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| Exact Mass |
324.1474
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| CAS # |
129-20-4
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| Related CAS # |
Oxyphenbutazone monohydrate;7081-38-1;Oxyphenbutazone-d9;1189693-23-9;Oxyphenbutazone-13C6
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| Appearance |
Typically exists as solids (or liquids in special cases) at room temperature
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| SMILES |
CCCCC1C(=O)N(C2C=CC=CC=2)N(C2C=CC(O)=CC=2)C1=O
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| Synonyms |
G 29701. Oxyphenbutazone; G29701; G-29701
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ~100 mg/mL (~308.29 mM)
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|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (7.71 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (7.71 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. View More
Solubility in Formulation 3: ≥ 2.5 mg/mL (7.71 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 3.0828 mL | 15.4140 mL | 30.8280 mL | |
| 5 mM | 0.6166 mL | 3.0828 mL | 6.1656 mL | |
| 10 mM | 0.3083 mL | 1.5414 mL | 3.0828 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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