| Size | Price | Stock | Qty |
|---|---|---|---|
| 5mg |
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| 10mg |
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| Other Sizes |
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| ln Vitro |
Panaxadiol (1-10 μM, 12 hours) suppresses PD-L1 expression in human colon cancer cells [1]. Panaxadiol (1-10 μM, 12 h) decreases PD-L1 expression by reducing HIF-1α and STAT3 in HCT116 cells [1].
|
|---|---|
| ln Vivo |
In xenograft models, panaxadiol (oral gavage; 10 or 30 mg/kg; every 3 days; 30 days) suppresses HCT116 cell proliferation [1].
|
| Cell Assay |
Western Blot Analysis[1]
Cell Types: HCT116, SW620 and HT-29 colon cancer cells Tested Concentrations: 1, 3 and 10 μM Incubation Duration: 12 hrs (hours) Experimental Results: The expression of PD-L1 protein and mRNA diminished in a dose-dependent manner. Western Blot Analysis[1] Cell Types: HCT116 Cell Tested Concentrations: 1, 3 and 10 μM Incubation Duration: 12 hrs (hours) Experimental Results: Inhibition of hypoxia-induced nuclear accumulation of HIF-1α in a dose-dependent manner. Inhibits STAT3 Tyr705 phosphorylation in a dose-dependent manner under both normoxic and hypoxic conditions. |
| Animal Protocol |
Animal/Disease Models: BALB/c athymic nude mice injected with HCT116 cells [1]
Doses: 10 or 30 mg/kg Route of Administration: po (oral gavage); 10 or 30 mg/kg; once every 3 days; 30-day Experimental Results: based on dose Dependent manner inhibits the protein levels of HIF-1α, p-STAT-3 (Tyr705), PD-L1 and VEGF in tumor tissues. |
| References |
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| Additional Infomation |
Panaxadiol is a triterpenoid saponin.
Panaxadiol has been reported in Panax ginseng with data available. |
| Molecular Formula |
C30H52O3
|
|---|---|
| Molecular Weight |
460.7321
|
| Exact Mass |
460.391
|
| CAS # |
19666-76-3
|
| PubChem CID |
73498
|
| Appearance |
White to off-white solid powder
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| Density |
1.0±0.1 g/cm3
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| Boiling Point |
531.3±45.0 °C at 760 mmHg
|
| Melting Point |
247 °C
|
| Flash Point |
275.1±28.7 °C
|
| Vapour Pressure |
0.0±3.2 mmHg at 25°C
|
| Index of Refraction |
1.515
|
| LogP |
7.64
|
| Hydrogen Bond Donor Count |
2
|
| Hydrogen Bond Acceptor Count |
3
|
| Rotatable Bond Count |
1
|
| Heavy Atom Count |
33
|
| Complexity |
789
|
| Defined Atom Stereocenter Count |
10
|
| SMILES |
C[C@@]1(CCCC(O1)(C)C)[C@H]2CC[C@@]3([C@@H]2[C@@H](C[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)O)C)C)O)C
|
| InChi Key |
PVLHOJXLNBFHDX-XHJPDDKBSA-N
|
| InChi Code |
InChI=1S/C30H52O3/c1-25(2)13-9-14-30(8,33-25)19-10-16-29(7)24(19)20(31)18-22-27(5)15-12-23(32)26(3,4)21(27)11-17-28(22,29)6/h19-24,31-32H,9-18H2,1-8H3/t19-,20+,21-,22+,23-,24-,27-,28+,29+,30+/m0/s1
|
| Chemical Name |
(3S,5R,8R,9R,10R,12R,13R,14R,17S)-4,4,8,10,14-pentamethyl-17-[(2R)-2,6,6-trimethyloxan-2-yl]-2,3,5,6,7,9,11,12,13,15,16,17-dodecahydro-1H-cyclopenta[a]phenanthrene-3,12-diol
|
| HS Tariff Code |
2934.99.9001
|
| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
DMSO : ~100 mg/mL (~217.05 mM)
Ethanol : ~20 mg/mL (~43.41 mM) |
|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (5.43 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (5.43 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.1705 mL | 10.8523 mL | 21.7047 mL | |
| 5 mM | 0.4341 mL | 2.1705 mL | 4.3409 mL | |
| 10 mM | 0.2170 mL | 1.0852 mL | 2.1705 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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