Size | Price | Stock | Qty |
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100mg |
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Other Sizes |
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ln Vitro |
Advice (The procedure we suggest is as follows. This protocol should be adjusted to suit your unique requirements; it merely offers guidelines. Compute: Use a > 12-fold molar excess of biotin for protein solutions containing 10 mg/mL and ≥ 20-fold molar excess for protein solutions containing 2 mg/mL for the best outcomes. Determine how many millimoles of the biotin reagent were added to the reaction. Twenty times is the molar excess: The formula for mmoL biotin is mL protein × (mg protein/mL protein) × (mmoL protein/mg protein) × (20 mmoL protein/mmoL protein). * 20 = 2 milligrams per milliliter What molar excess of biotin in a protein sample is recommended? 2. Determine the quantity of 10 mM biotin reagent solution microliters (made in step B.3) that needs to be added to the reaction: µL biotin is equal to mmoL biotin × (1000000 µL/L) × (L/ 10 mmoL). Reaction of biotin labeling: 1–10 mg of protein should be dissolved in 0.5–2.0 mL of PBS using the computation in Calculation. 2. Make a 10 mM biotin reagent solution in an organic solvent (like DMSO) before using. 3. To the protein solution, add the appropriate volume (μL) of the 10 mM biotin reagent solution. 4. Let the reaction sit on ice for two hours or for half an hour at room temperature. 5. Use Western blotting or ELISA to find tagged proteins.
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References |
[1]. Wojchowski DM et al. Hybridoma production by simplified avidin-mediated electrofusion. J Immunol Methods. 1986 Jun 24;90(2):173-7.
[2]. Ngo TT et al. Biotinyl-glucose-6-phosphate dehydrogenase preparation, kinetics, and modulation by avidin. Appl Biochem Biotechnol. 1982 Nov;7(6):443-54. |
Molecular Formula |
C14H19N3O5S
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Molecular Weight |
341.3828
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CAS # |
35013-72-0
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Appearance |
Typically exists as solids (or liquids in special cases) at room temperature
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SMILES |
S1C([H])([H])[C@@]2([H])[C@@]([H])([C@]1([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C(=O)ON1C(C([H])([H])C([H])([H])C1=O)=O)N([H])C(N2[H])=O
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HS Tariff Code |
2934.99.9001
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Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: (1). Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture and light. (2). This product is not stable in solution, please use freshly prepared working solution for optimal results. |
Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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Solubility (In Vitro) |
DMSO : ~62.5 mg/mL (~183.08 mM)
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Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.08 mg/mL (6.09 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.08 mg/mL (6.09 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C,1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. View More
Solubility in Formulation 3: ≥ 2.08 mg/mL (6.09 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. |
Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
1 mM | 2.9293 mL | 14.6464 mL | 29.2929 mL | |
5 mM | 0.5859 mL | 2.9293 mL | 5.8586 mL | |
10 mM | 0.2929 mL | 1.4646 mL | 2.9293 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration: mg/mL;
Method for preparing DMSO stock solution: mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation::Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O,mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.