| Size | Price | Stock | Qty |
|---|---|---|---|
| 5mg |
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| 10mg |
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| 50mg |
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| Other Sizes |
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| ln Vitro |
Normal colon cell proliferation is not significantly inhibited by pinosinbene (0–40 μM; 24–48 hours) [1]. When compared to control cells, pinostilbene (20 μM, 40 μM; 24 hours, 48 hours) significantly and dose-dependently increases the percentage of S phase cells in HCT116 and HT29 cells [1]. A significant increase in the number of G2/M phase cells in HT29 cells can also be induced by μM of pine stilbene [1]. Pinostilbene (20 μM, 40 μM; 24 h, 48 h) controls the expression of important signaling proteins linked to both apoptosis and cell growth [1]. In addition, pinosinbene functions as a methylated resveratrol derivative and shields SH-SY5Y cells from the neurotoxicity caused by 6-hydroxydopamine [2].
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| Cell Assay |
Cell viability assay [1]
Cell Types: HCT116 cells, HT29 cells Tested Concentrations: 24 hrs (hours), 48 hrs (hours) Incubation Duration: 0-100 μM Experimental Results: Inhibited the growth of two human colon cancer cells. Apoptosis analysis [1] Cell Types: HCT116 cells, HT29 cells Tested Concentrations: 20 μM, 40 μM Incubation Duration: 24 hrs (hours), 48 hrs (hours) Experimental Results: resulted in a significant and dose-dependent increase in the percentage of cells in S phase. Cell viability assay [1] Cell Types: HCT116 Cell Tested Concentrations: 20 μM, 40 μM Incubation Duration: 24 hrs (hours), 48 hrs (hours) Experimental Results: The expression levels of p53, Bax, cleaved caspase-3, cleaved PARP and p21Cip1/Waf1 increased Dramatically, At the same time, the expression levels of cyclin E and p-Rb were diminished. |
| References |
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| Additional Infomation |
3-methoxy-4',5-dihydroxy-trans-stilbene is a stilbenoid that is trans-resveratrol in which one of the meta-hydroxy groups is converted to the corresponding methyl ether. It is functionally related to a trans-resveratrol.
3-Methoxy-4',5-dihydroxy-trans-stilbene has been reported in Soymida febrifuga, Streptomyces avermitilis, and other organisms with data available. |
| Molecular Formula |
C15H14O3
|
|---|---|
| Molecular Weight |
242.2699
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| Exact Mass |
242.094
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| CAS # |
42438-89-1
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| PubChem CID |
5473050
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| Appearance |
Off-white to light yellow solid powder
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| Density |
1.3±0.1 g/cm3
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| Boiling Point |
454.3±33.0 °C at 760 mmHg
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| Melting Point |
117-118℃
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| Flash Point |
228.5±25.4 °C
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| Vapour Pressure |
0.0±1.2 mmHg at 25°C
|
| Index of Refraction |
1.692
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| LogP |
3.8
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| Hydrogen Bond Donor Count |
2
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| Hydrogen Bond Acceptor Count |
3
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| Rotatable Bond Count |
3
|
| Heavy Atom Count |
18
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| Complexity |
269
|
| Defined Atom Stereocenter Count |
0
|
| SMILES |
COC1=CC(=CC(=C1)O)/C=C/C2=CC=C(C=C2)O
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| InChi Key |
KUWZXOMQXYWKBS-NSCUHMNNSA-N
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| InChi Code |
InChI=1S/C15H14O3/c1-18-15-9-12(8-14(17)10-15)3-2-11-4-6-13(16)7-5-11/h2-10,16-17H,1H3/b3-2+
|
| Chemical Name |
3-[(E)-2-(4-hydroxyphenyl)ethenyl]-5-methoxyphenol
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ~33.33 mg/mL (~137.57 mM)
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|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (10.32 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (10.32 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. View More
Solubility in Formulation 3: ≥ 2.5 mg/mL (10.32 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 4.1276 mL | 20.6381 mL | 41.2763 mL | |
| 5 mM | 0.8255 mL | 4.1276 mL | 8.2553 mL | |
| 10 mM | 0.4128 mL | 2.0638 mL | 4.1276 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
Products are for research use only; We do not sell to patients
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