| Size | Price | Stock | Qty |
|---|---|---|---|
| 100mg |
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| Other Sizes |
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| ln Vitro |
The cytotoxic effects of pinosylvin (0-100 μM) on THP-1 and U937 cells are observed after 24 hours of incubation at 100 μM. In leukemia cells, pinosylvin exhibits IC50 values of 20-30 μM. At 50 μM and 100 μM, respectively, pinosylvin (0-100 μM; 24 hours) increases the number of Annexin V+ and PI+ cells in the U937 population and the THP-1 population [3]. By increasing the levels of LC3-II and p62/SQSTM1 degradation in leukemia cells, pinosylvin (0-100 μM; 24 hours) stimulates autophagy in leukemia cells [3].
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|---|---|
| ln Vivo |
Sonacillin (iv; 10 mg/kg) produced plasma AUC, urine t, CL, and Vd values of 5.23 ± 1.20 mgh mL-1, 13.13 ± 2.05 h, 1.84 ± 0.44 Lh-1kg-1, and 2.29 Lkg-1 , PK experiments were conducted in male Sprague-Dawley rats [1].
|
| Cell Assay |
Cell viability assay [3]
Cell Types: Leukemia cells Tested Concentrations: 0 μM; 0.1 μM; 1 μM; 10 μM; 50 μM; 100 μM Incubation Duration: 24 hrs (hours) Experimental Results: Cytotoxic to leukemia cells at high concentrations. Apoptosis analysis [3] Cell Types: U937 and THP-1 Cell Tested Concentrations: 0 μM; 0.1 μM; 1 μM; 10 μM; 50 μM; 100 μM Incubation Duration: 24 hrs (hours) Experimental Results: Apoptosis induced in U937 and THP-1 cells Number of cells. Western Blot Analysis [3] Cell Types: U937 and THP-1 Cell Tested Concentrations: 0 μM; 0.1 μM; 1 μM; 10 μM; 50 μM; 100 μM Incubation Duration: 24 hrs (hours) Experimental Results: Induction of autophagy in leukemia cells. |
| References |
|
| Additional Infomation |
Pinosylvin is a stilbenol.
Pinosylvin has been reported in Pinaceae, Pinus pinea, and other organisms with data available. |
| Molecular Formula |
C14H12O2
|
|---|---|
| Molecular Weight |
212.2439
|
| Exact Mass |
212.083
|
| CAS # |
22139-77-1
|
| PubChem CID |
5280457
|
| Appearance |
White to off-white solid powder
|
| Density |
1.3±0.1 g/cm3
|
| Boiling Point |
397.6±17.0 °C at 760 mmHg
|
| Melting Point |
155.5-156°
|
| Flash Point |
194.9±15.5 °C
|
| Vapour Pressure |
0.0±1.0 mmHg at 25°C
|
| Index of Refraction |
1.727
|
| LogP |
3.68
|
| Hydrogen Bond Donor Count |
2
|
| Hydrogen Bond Acceptor Count |
2
|
| Rotatable Bond Count |
2
|
| Heavy Atom Count |
16
|
| Complexity |
221
|
| Defined Atom Stereocenter Count |
0
|
| SMILES |
C1=CC=C(C=C1)/C=C/C2=CC(=CC(=C2)O)O
|
| InChi Key |
YCVPRTHEGLPYPB-VOTSOKGWSA-N
|
| InChi Code |
InChI=1S/C14H12O2/c15-13-8-12(9-14(16)10-13)7-6-11-4-2-1-3-5-11/h1-10,15-16H/b7-6+
|
| Chemical Name |
5-[(E)-2-phenylethenyl]benzene-1,3-diol
|
| HS Tariff Code |
2934.99.9001
|
| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
DMSO : ~100 mg/mL (~471.16 mM)
|
|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (11.78 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (11.78 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. View More
Solubility in Formulation 3: ≥ 2.5 mg/mL (11.78 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 4.7116 mL | 23.5582 mL | 47.1165 mL | |
| 5 mM | 0.9423 mL | 4.7116 mL | 9.4233 mL | |
| 10 mM | 0.4712 mL | 2.3558 mL | 4.7116 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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