| Size | Price | Stock | Qty |
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| 50mg |
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| 100mg |
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| 250mg |
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| 500mg |
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| Other Sizes |
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| Targets |
MSSA(MIC= 0.3 µM);MRSA(MIC= 0.3-1µM);VISA(MIC= 0.3 µM)
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| ln Vivo |
Using a mouse bloodstream infection model, the in vivo effectiveness of PK150 against methicillin-sensitive S. aureus (MSSA) (strain SH1000) is shown. The liver and heart have much lower bacterial loads when treated with PK150 (20 mg/kg; p.o.).In mice, PK150 (10 and 20 mg/kg orally; or 10 mg/kg intravenously) does not manifest any overt toxicity. For later therapeutic models, higher intravenous dosages of 20 mg/kg are avoided due to their severe toxic effects[1].
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| Animal Protocol |
Animal Model: Pathogen-free 9-week old female C57BL/6J mice[1]
Dosage: 20 mg/kg Administration: Administered p.o. Result: Both the liver and the heart had markedly decreased bacterial loads, which had decreased by about 100 times. |
| References |
| Molecular Formula |
C15H8CLF5N2O3
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|---|---|
| Molecular Weight |
394.68
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| CAS # |
2165324-62-7
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| Appearance |
Typically exists as solids (or liquids in special cases) at room temperature
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| SMILES |
ClC1C=CC(=CC=1C(F)(F)F)NC(NC1C=CC2=C(C=1)OC(O2)(F)F)=O
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| InChi Key |
SHPYXCLUNMOJOB-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C15H8ClF5N2O3/c16-10-3-1-7(5-9(10)14(17,18)19)22-13(24)23-8-2-4-11-12(6-8)26-15(20,21)25-11/h1-6H,(H2,22,23,24)
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| Chemical Name |
1-[4-chloro-3-(trifluoromethyl)phenyl]-3-(2,2-difluoro-1,3-benzodioxol-5-yl)urea
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| Synonyms |
PK-150
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ~250 mg/mL (~633.42 mM)
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| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.08 mg/mL (5.27 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.08 mg/mL (5.27 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.5337 mL | 12.6685 mL | 25.3370 mL | |
| 5 mM | 0.5067 mL | 2.5337 mL | 5.0674 mL | |
| 10 mM | 0.2534 mL | 1.2668 mL | 2.5337 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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