| Size | Price | Stock | Qty |
|---|---|---|---|
| 1g |
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| 5g |
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| Other Sizes |
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| ln Vitro |
The hydrolysis of PNPP is catalyzed by phosphatase, which releases inorganic phosphate and p-nitrophenol's conjugate base (pNP). The resultant phenolate has a maximum absorption wavelength of 405 nm and is yellow in color[1].
|
|---|---|
| References |
| Molecular Formula |
C6H4NNA2O6P
|
|---|---|
| Molecular Weight |
263.0524
|
| Exact Mass |
218.993
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| CAS # |
4264-83-9
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| Related CAS # |
330-13-2 (Parent)
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| PubChem CID |
77949
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| Appearance |
White to yellow solid powder
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| Density |
1.7±0.1 g/cm3
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| Boiling Point |
457.8±47.0 °C at 760 mmHg
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| Melting Point |
>300 °C(lit.)
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| Flash Point |
230.7±29.3 °C
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| Vapour Pressure |
0.0±1.2 mmHg at 25°C
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| Index of Refraction |
1.617
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| LogP |
0.37
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| Hydrogen Bond Donor Count |
0
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| Hydrogen Bond Acceptor Count |
6
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| Rotatable Bond Count |
1
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| Heavy Atom Count |
16
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| Complexity |
238
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| Defined Atom Stereocenter Count |
0
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| InChi Key |
VIYFPAMJCJLZKD-UHFFFAOYSA-L
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| InChi Code |
InChI=1S/C6H6NO6P.2Na/c8-7(9)5-1-3-6(4-2-5)13-14(10,11)12;;/h1-4H,(H2,10,11,12);;/q;2*+1/p-2
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| Chemical Name |
disodium;(4-nitrophenyl) phosphate
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture and light. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
H2O : ~160 mg/mL (~608.25 mM)
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|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: 100 mg/mL (380.16 mM) in PBS (add these co-solvents sequentially from left to right, and one by one), clear solution; with sonication.
(Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 3.8016 mL | 19.0078 mL | 38.0156 mL | |
| 5 mM | 0.7603 mL | 3.8016 mL | 7.6031 mL | |
| 10 mM | 0.3802 mL | 1.9008 mL | 3.8016 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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