Size | Price | |
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500mg | ||
1g | ||
Other Sizes |
ln Vitro |
By preventing oxidative stress in vitro, procaterol reduces inflammation [2]. 16HBE cell viability can be increased by procaterol (25–200 nM) [2].
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ln Vivo |
The freezing levels elicited by conditioned stimulus (CS) are dramatically increased by procaterol [3]. The behavioral expression of Pavlovian threat conditioning (PTC) was dramatically reduced when beta-adrenergic receptors (β-AR) were blocked by propranolol, while PTC was enhanced when β2-AR was activated systemically with procaterol [3]. Procaterol hydrochloride should be freshly dissolved in 0.9% sterile saline before injection [3].
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Cell Assay |
Cell viability assay [2]
Cell Types: Immortal human bronchial epithelial cell line (16HBE) Tested Concentrations: 25-200 nM Incubation Duration: Pretreatment for 20 hrs (hours) Experimental Results: Pretreatment increased the cell viability rate in a dose-dependent manner. Pretreatment with procaterol combined with dexamethasone can also Dramatically improve cell survival rate, but the combined effect is not better than pretreatment with procaterol alone at the same dose. |
Animal Protocol |
Animal/Disease Models: Adult male SD (SD (Sprague-Dawley)) rat, body weight 250-275 g (approximately 60-70 days old) [3]
Doses: 300 µg/kg Route of Administration: intraperitoneal (ip) injection Experimental Results: Systemic injection of specific β2 -The AR agonist procaterol (300 µg/kg) enhanced CS-induced freezing during the expression test but had no effect during the drug-free test. |
References |
[1]. C Atgié, et al. Role of beta1- and beta3-adrenoceptors in the regulation of lipolysis and thermogenesis in rat brown adipocytes. Am J Physiol. 1997 Oct;273(4):C1136-42.
[2]. Zheng Deng, et al. Procaterol but not dexamethasone protects 16HBE cells from H2O2-induced oxidative stress. J Pharmacol Sci. 2014;125(1):39-50. [3]. Yiran Gu, et al. A brainstem-central amygdala circuit underlies defensive responses to learned threats. Mol Psychiatry. 2020 Mar;25(3):640-654. |
Additional Infomation |
Procaterol hydrochloride is an organic molecular entity.
A long-acting beta-2-adrenergic receptor agonist. See also: Procaterol Hydrochloride (annotation moved to). |
Molecular Formula |
C16H22N2O3.HCL
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Molecular Weight |
326.81842
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Exact Mass |
326.139
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CAS # |
62929-91-3
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Related CAS # |
Procaterol hydrochloride hemihydrate;81262-93-3;(Rac)-Procaterol hydrochloride;59828-07-8
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PubChem CID |
656601
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Appearance |
Typically exists as solid at room temperature
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Boiling Point |
539.5ºC at 760 mmHg
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Flash Point |
280.1ºC
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LogP |
3.236
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Hydrogen Bond Donor Count |
5
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Hydrogen Bond Acceptor Count |
4
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Rotatable Bond Count |
5
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Heavy Atom Count |
22
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Complexity |
397
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Defined Atom Stereocenter Count |
0
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SMILES |
Cl.CC(N[C@@H]([C@H](C1C=CC(O)=C2NC(C=CC=12)=O)O)CC)C
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InChi Key |
AEQDBKHAAWUCMT-UHFFFAOYSA-N
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InChi Code |
InChI=1S/C16H22N2O3.ClH/c1-4-12(17-9(2)3)16(21)11-5-7-13(19)15-10(11)6-8-14(20)18-15;/h5-9,12,16-17,19,21H,4H2,1-3H3,(H,18,20);1H
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Chemical Name |
8-hydroxy-5-[1-hydroxy-2-(propan-2-ylamino)butyl]-1H-quinolin-2-one;hydrochloride
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HS Tariff Code |
2934.99.9001
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Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
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Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80: Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 :Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400  (Please use freshly prepared in vivo formulations for optimal results.) |
Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
1 mM | 3.0598 mL | 15.2989 mL | 30.5979 mL | |
5 mM | 0.6120 mL | 3.0598 mL | 6.1196 mL | |
10 mM | 0.3060 mL | 1.5299 mL | 3.0598 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration: mg/mL;
Method for preparing DMSO stock solution: mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation::Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O,mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.