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ln Vitro |
Phoyunnanin E (~10 μM; 48 hours; H460 cells) suppresses migration of cells by downregulating integrins[1]. At 10 μM at 48 hours, phoyunnanin E (0~100 μM; 24 and 48 hours; H460 cells) significantly reduces the proliferation rate of treated cells in a dose-dependent manner[1]. Phoyunnanin E (0~100 μM; 24 hours; H460 cells) causes a marked rise in necrotic and apoptotic cells at 20~100 μM and cytotoxic effects at concentrations between 50 and 100 μM[1]. The number of cells invading across the matrix and transwell filter within 24 hours is significantly reduced by phoyunnanin E (0~10 μM; 48 hours; H460, H292, and A549 cells) in a dose-dependent manner[1]. Lung cancer H460 cells' growth and migration are inhibited by phoyunnanin E, even in the absence of anchorage. At concentrations of 5 and 10 μM, phoyunnanin E significantly reduces cell migration across the wound space at 24 and 48 hours when compared to the untreated control. At the protrusion edges of the cells, phoyunnanin E (0~10 μM; 48 hours) significantly reduces the number of filopodia in a dose-dependent manner. In additional human lung cancer cases, phoyunnanin E reduces cancer cell invasion and migration. Inhibiting the epithelial-mesenchymal transition (EMT) is phoyunnanin E. In cell migration, integrins αv and β3, as well as the regulatory proteins, are down-regulated by phoyunnanin E[1].
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Cell Assay |
Western Blot Analysis[1]
Cell Types: H460 cells Tested Concentrations: 1~10 μM Incubation Duration: 48 hrs (hours) Experimental Results: diminished cell migration through integrin downregulation. Cell Proliferation Assay[1] Cell Types: H460 cells Tested Concentrations: 0~100 μM Incubation Duration: 24 and 48 hrs (hours) Experimental Results: The proliferation rate of treated cells had Dramatically diminished in a dose-dependent manner at the concentration of 10 μM at 48 h. Apoptosis Analysis[1] Cell Types: H460 cells Tested Concentrations: 0~100 μM Incubation Duration: 24 hrs (hours) Experimental Results: Induced a significant increase in apoptotic and necrotic cells at 20~100 μM. Cell Cytotoxicity Assay[1] Cell Types: H460 cells Tested Concentrations: 0~100 μM Incubation Duration: 24 hrs (hours) Experimental Results: Induced cytotoxic effects at the concentrations from 50 to 100 μM. Immunofluorescence[1] Cell Types: H460, H292 and A549 cells Tested Concentrations: 0~10 μM Incubation Duration: 48 hrs (hours) Experimental Results: Dramatically diminished the number of cells invading across the matrix and transwell filter within 24 hrs (hours) in a dose-de |
References |
[1]. Petpiroon N, et al. Phoyunnanin E inhibits migration of non-small cell lung cancer cells via suppression of epithelial-to-mesenchymal transition and integrin αv and integrin β3. BMC Complement Altern Med. 2017;17(1):553. Published 2017 Dec 29.
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Molecular Formula |
C30H26O6
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Molecular Weight |
482.52
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CAS # |
886747-60-0
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Appearance |
Typically exists as solids (or liquids in special cases) at room temperature
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SMILES |
OC1C=C2CCC3C(C2=CC=1)=C(O)C=C(OC)C=3OC1C=C2CCC3C(C2=CC=1)=C(O)C=C(OC)C=3
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HS Tariff Code |
2934.99.9001
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Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
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Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80: Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 :Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400  (Please use freshly prepared in vivo formulations for optimal results.) |
Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
1 mM | 2.0725 mL | 10.3623 mL | 20.7245 mL | |
5 mM | 0.4145 mL | 2.0725 mL | 4.1449 mL | |
10 mM | 0.2072 mL | 1.0362 mL | 2.0725 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration: mg/mL;
Method for preparing DMSO stock solution: mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation::Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O,mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.