Size | Price | Stock | Qty |
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1mg |
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Other Sizes |
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ln Vitro |
In neural progenitor cells (NPCs), (+)-intermedine (1.1-30 μM; 24 hours) shows notable cytotoxicity at 30 μM and decreases cell viability in a concentration-dependent manner[1]. (+)-Intermedine exhibits no deficiency in differentiation[1].
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Cell Assay |
Cell Cytotoxicity Assay[1]
Cell Types: Neural progenitor cells (NPCs) Tested Concentrations: 1.1, 3.3, 10, 30 μM Incubation Duration: 24 hrs (hours) Experimental Results: demonstrated significant cytotoxicity at 30 μM and decreased cell viability in a concentration dependent manner. |
References |
Molecular Formula |
C15H25NO5
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Molecular Weight |
299.36
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Exact Mass |
299.173
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CAS # |
10285-06-0
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Appearance |
White to off-white solid
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Density |
1.26g/cm3
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Boiling Point |
463.3ºC at 760mmHg
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Flash Point |
234ºC
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Vapour Pressure |
1.57E-10mmHg at 25°C
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Index of Refraction |
1.567
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LogP |
-0.4
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SMILES |
CC([C@](C(OCC1=CCN2CC[C@@H](O)[C@@H]12)=O)(O)[C@H](O)C)C
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InChi Key |
SFVVQRJOGUKCEG-OPQSFPLASA-N
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InChi Code |
InChI=1S/C15H25NO5/c1-9(2)15(20,10(3)17)14(19)21-8-11-4-6-16-7-5-12(18)13(11)16/h4,9-10,12-13,17-18,20H,5-8H2,1-3H3/t10-,12-,13-,15+/m1/s1
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Chemical Name |
[(7R,8R)-7-hydroxy-5,6,7,8-tetrahydro-3H-pyrrolizin-1-yl]methyl (2S)-2-hydroxy-2-[(1R)-1-hydroxyethyl]-3-methylbutanoate
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Synonyms |
(+)-Intermedine
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HS Tariff Code |
2934.99.9001
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Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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Solubility (In Vitro) |
DMSO: 50 mg/mL (167.02 mM)
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Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 1.25 mg/mL (4.18 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 12.5 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C,1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. Solubility in Formulation 2: ≥ 1.25 mg/mL (4.18 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 12.5 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly.  (Please use freshly prepared in vivo formulations for optimal results.) |
Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
1 mM | 3.3405 mL | 16.7023 mL | 33.4046 mL | |
5 mM | 0.6681 mL | 3.3405 mL | 6.6809 mL | |
10 mM | 0.3340 mL | 1.6702 mL | 3.3405 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration: mg/mL;
Method for preparing DMSO stock solution: mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation::Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O,mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.