Size | Price | Stock | Qty |
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100mg |
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Other Sizes |
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ln Vitro |
Only a little residual signal of the POBN radical adduct is seen when POBN (20 mM), sodium formate (100 mM), and bile are combined in the collecting tube with 2,2′-dipyridyl (DP) (5 mM) and bathocuproinedisulfonic acid disodium salt hydrate (BC) (5 mM)[1].
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ln Vivo |
Rats' bile contained radical adducts identified by electron spin resonance (ESR) spectra one hour following acute intraperitoneal (i.p.) administration of sodium formate (2 g/kg) and POBN (1.5 g/kg)[1].
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Animal Protocol |
Animal/Disease Models: Fischer male rats (300- 400 g)[1]
Doses: 1.5 g/kg (pharmacokinetic/PK Analysis) Route of Administration: Injected simultaneously ip Experimental Results: A strong six-line ESR signal of the POBN radical adduct was detected in the bile of rats after acute sodium formate poisoning. |
References |
[1]. A E Dikalova, et al. An in vivo ESR spin-trapping study: free radical generation in rats from formate intoxication--role of the Fenton reaction. Proc Natl Acad Sci U S A. 2001 Nov 20;98(24):13549-53.
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Molecular Formula |
C10H14N2O2
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Molecular Weight |
194.23
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CAS # |
66893-81-0
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Appearance |
Typically exists as solids (or liquids in special cases) at room temperature
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SMILES |
[O-]/[N+](=C(/[H])\C1C([H])=C([H])[N+](=C([H])C=1[H])[O-])/C(C([H])([H])[H])(C([H])([H])[H])C([H])([H])[H]
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HS Tariff Code |
2934.99.9001
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Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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Solubility (In Vitro) |
H2O: 100 mg/mL (514.85 mM)
DMSO: 50 mg/mL (257.43 mM) |
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Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (12.87 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (12.87 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C,1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. View More
Solubility in Formulation 3: ≥ 2.5 mg/mL (12.87 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. Solubility in Formulation 4: 100 mg/mL (514.85 mM) in PBS (add these co-solvents sequentially from left to right, and one by one), clear solution; with ultrasonication. |
Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
1 mM | 5.1485 mL | 25.7427 mL | 51.4854 mL | |
5 mM | 1.0297 mL | 5.1485 mL | 10.2971 mL | |
10 mM | 0.5149 mL | 2.5743 mL | 5.1485 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration: mg/mL;
Method for preparing DMSO stock solution: mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation::Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O,mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.