| Size | Price | Stock | Qty |
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| 1mg |
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| 5mg |
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| 10mg |
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| Other Sizes |
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| Targets |
IC50: 63.06 nM (CXXC5-DVL)[1]
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| ln Vitro |
Through the Wnt/β-eatenin signaling pathway, A3334 (1–10 μM) effectively prevents 3T3-L1 preadipocytes from differentiating into adipocytes[1]. In HEK293-TOP cells, A3334 (1–10 μM; 24 h) increases TOPFlash reporter activity [1].
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| ln Vivo |
In mice on a high-fat diet (HFD), A3334 (25 mg/kg; po once daily for 16 weeks) exhibits anti-obesity benefits; in mice fed a regular diet, it has no effect[1]. A3334 (25 mg/kg; po once daily for 5 days) dramatically lowers blood levels of insulin tolerance (ITT) and glucose tolerance (GTT) in mice as well as fasting glucose[1]. In mice, hepatosteatosis and elevated levels of alanine transaminase (ALT) and aspartate transaminase (AST) are markedly reduced by A3334 (25 mg/kg; po once daily for 3 weeks)[1].
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| Animal Protocol |
Animal/Disease Models: Male C57BL/6N mice (6weeks old) are fed on the high fat die (HFD) for 16 weeks[1]
Doses: 25 mg/kg Route of Administration: Po daily for 16 weeks Experimental Results: Not observed the HFD-induced body weight gain and abdominal obesity. decreased the level of triglyceride and total cholesterol and increased the level of HDL-cholesterol. Suppressed the increase in adipocyte cell sizes and enhancement of inflammation. |
| References |
[1]. Choi KY, et, al. Compositions and methods for suppressing and/or treating metabolic diseases and/or a clinical condition thereof. WO2020079569.
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| Molecular Formula |
C17H13N3O3
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|---|---|
| Molecular Weight |
307.30
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| CAS # |
854171-31-6
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| Appearance |
Typically exists as solids (or liquids in special cases) at room temperature
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| SMILES |
O([H])C1=C(C2C([H])=C(C([H])=C([H])C=2N1[H])OC([H])([H])[H])C1=C(C2=C([H])C([H])=C([H])C([H])=C2N1[H])N=O
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO: 250 mg/mL (813.54 mM)
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|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.08 mg/mL (6.77 mM) (saturation unknown) in 10% DMSO + 40% PEG300 +5% Tween-80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 + to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 3.2541 mL | 16.2707 mL | 32.5415 mL | |
| 5 mM | 0.6508 mL | 3.2541 mL | 6.5083 mL | |
| 10 mM | 0.3254 mL | 1.6271 mL | 3.2541 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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