| Size | Price | Stock | Qty |
|---|---|---|---|
| 5mg |
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| 10mg |
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| 50mg |
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| 100mg |
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| Other Sizes |
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| ln Vitro |
After being transfected with pMaRSC and treated for six hours with 1.5 mM of L-Azidonorleucine hydrochloride (AnI), CHO cells were permeabilized, fixed, and given alkyne-TAMRA treatment. Anl cells selectively incorporated cellular proteins, as evidenced by the observation of TAMRA fluorescence exclusively in mCherry-expressing cells [1].
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|---|---|
| ln Vivo |
To label and identify neuronal populations in the brain, an average daily intake of L-Azidonorleucine (ANL) of 0.9 mg/day/g body weight over a period of 21 days is advised when ANL is delivered through drinking water. Other cell types can be labeled with lower doses or shorter labeling times. It is advised to experiment with various intraperitoneal injection doses of L-azidonorleucine within the 4-400 mM range. It is advised to use intraperitoneal injection; to label specific neuronal populations (such as the excitatory neurons in the hippocampus), a 400 mM dose (ip volume: 10 ml/kg) should be administered once daily for one week. When labeling different cell types or when longer labeling times are needed, lower doses—such as 4 mM—may be used [2].
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| References |
[1]. Alborz Mahdavi, et al. Engineered Aminoacyl-tRNA Synthetase for Cell-Selective Analysis of Mammalian Protein Synthesis. J Am Chem Soc. 2016 Apr 6;138(13):4278-81.
[2]. Beatriz Alvarez-Castelao, et al. Cell-type-specific metabolic labeling, detection and identification of nascent proteomes in vivo. Nat Protoc. 2019 Feb;14(2):556-575. |
| Molecular Formula |
C6H13CLN4O2
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|---|---|
| Molecular Weight |
208.65
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| CAS # |
1454334-76-9
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| Appearance |
Typically exists as solids (or liquids in special cases) at room temperature
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| SMILES |
Cl.OC([C@H](CCCCN=[N+]=[N-])N)=O
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment, avoid exposure to moisture. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
H2O: 125 mg/mL (599.09 mM)
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|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: 100 mg/mL (479.27 mM) in PBS (add these co-solvents sequentially from left to right, and one by one), clear solution; with sonication.
(Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 4.7927 mL | 23.9636 mL | 47.9272 mL | |
| 5 mM | 0.9585 mL | 4.7927 mL | 9.5854 mL | |
| 10 mM | 0.4793 mL | 2.3964 mL | 4.7927 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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