| Size | Price | Stock | Qty |
|---|---|---|---|
| 100mg |
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| Other Sizes |
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| ln Vitro |
In cell culture and microbiology, TMPD (N,N,N′,N′-tetramethyl-para-phenylene-diamine) is used to discriminate between pathogenic and non-pathogenic bacteria as well as organisms that have cytochrome c oxidase activity. The oxidation of TMPD by microorganisms is a widely recognized concept in colorimetry for bacterial oxidases, due to the formation of the radical cation TMPD+, which exhibits a distinctive deep blue color. Numerous infections can be targeted by bacterial oxidases that electrochemically recognize the TMPD Oxidation[1].
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|---|---|
| References |
[1]. S Kuss, et al. Electrochemical recognition and quantification of cytochrome c expression in Bacillus subtilis and aerobe/anaerobe Escherichia coli using N, N, N', N'-tetramethyl- para-phenylene-diamine (TMPD). Chem Sci. 2017 Nov 1;8(11):7682-7688.
[2]. Nenad Petrovic, et al. Using N,N,N',N'-tetramethyl-p-phenylenediamine (TMPD) to assay cyclooxygenase activity in vitro. Methods Mol Biol. 2010;594:129-40. [3]. Franco A Rossato, et al. Fatty acid synthase inhibitors induce apoptosis in non-tumorigenic melan-a cells associated with inhibition of mitochondrial respiration. PLoS One. 2014 Jun 25;9(6):e101060. |
| Additional Infomation |
Used in the form of the hydrochloride as a reagent in ANALYTICAL CHEMISTRY TECHNIQUES.
|
| Molecular Formula |
C10H18CL2N2
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|---|---|
| Molecular Weight |
237.17
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| Exact Mass |
236.084
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| CAS # |
637-01-4
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| PubChem CID |
71561
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| Appearance |
Typically exists as solid at room temperature
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| Boiling Point |
260.6ºC at 760 mmHg
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| Melting Point |
222-224 °C(lit.)
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| Flash Point |
104.9ºC
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| LogP |
3.422
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| Hydrogen Bond Donor Count |
2
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| Hydrogen Bond Acceptor Count |
2
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| Rotatable Bond Count |
2
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| Heavy Atom Count |
14
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| Complexity |
108
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| Defined Atom Stereocenter Count |
0
|
| SMILES |
Cl[H].Cl[H].N(C([H])([H])[H])(C([H])([H])[H])C1C([H])=C([H])C(=C([H])C=1[H])N(C([H])([H])[H])C([H])([H])[H]
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| InChi Key |
FBHKTSXMTASXFJ-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C10H16N2.2ClH/c1-11(2)9-5-7-10(8-6-9)12(3)4;;/h5-8H,1-4H3;2*1H
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| Chemical Name |
1-N,1-N,4-N,4-N-tetramethylbenzene-1,4-diamine;dihydrochloride
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture and light. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
H2O: 100 mg/mL (421.64 mM)
DMSO: 16.67 mg/mL (70.29 mM) |
|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 1.67 mg/mL (7.04 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 16.7 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. Solubility in Formulation 2: 100 mg/mL (421.64 mM) in PBS (add these co-solvents sequentially from left to right, and one by one), clear solution; with ultrasonication. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 4.2164 mL | 21.0819 mL | 42.1638 mL | |
| 5 mM | 0.8433 mL | 4.2164 mL | 8.4328 mL | |
| 10 mM | 0.4216 mL | 2.1082 mL | 4.2164 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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