| Size | Price | |
|---|---|---|
| 500mg | ||
| 1g | ||
| Other Sizes |
| ln Vitro |
Poly(2-hydroxyethyl methacrylate) (PolyHEMA) deters host cell absorption and breakdown while maintaining a moisture content akin to that of living tissue. It is simple to mold into many shapes and simple to sterilize[1]. When cells were grown on poly(2-hydroxyethyl methacrylate), their rates of proliferation were decreased; in hRPE cultures, poly (2-hydroxyethyl methacrylate) did not cause cell death. Numerous enormous spheroid colonies were produced by human retinal pigment epithelial (RPE) cells grown on poly (2-hydroxyethyl methacrylate). After megacolonies were recultured, poly(2-hydroxyethyl methacrylate) cell cultures were shown to include markers for retinal progenitor cells and hRPE cells [1].
|
|---|---|
| References |
[1]. Fatemeh Nazemroaya, et al. Induced Retro-Differentiation of Human Retinal Pigment Epithelial Cells on PolyHEMA. J Cell Biochem. 2017 Oct;118(10):3080-3089.
|
| Additional Infomation |
A biocompatible, hydrophilic, inert gel that is permeable to tissue fluids. It is used as an embedding medium for microscopy, as a coating for implants and prostheses, for contact lenses, as microspheres in adsorption research, etc.
See also: Poly(2-hydroxyethyl methacrylate) (annotation moved to). |
| Molecular Formula |
C6H10O3
|
|---|---|
| Molecular Weight |
20000(Average)
|
| Exact Mass |
130.063
|
| CAS # |
25249-16-5
|
| PubChem CID |
18942519
|
| Appearance |
Typically exists as solid at room temperature
|
| Density |
1.054g/cm3
|
| Boiling Point |
189ºC at 760mmHg
|
| Flash Point |
97.2ºC
|
| Vapour Pressure |
0.159mmHg at 25°C
|
| Index of Refraction |
1.442
|
| LogP |
0.098
|
| Hydrogen Bond Donor Count |
1
|
| Hydrogen Bond Acceptor Count |
3
|
| Rotatable Bond Count |
5
|
| Heavy Atom Count |
11
|
| Complexity |
129
|
| Defined Atom Stereocenter Count |
0
|
| SMILES |
OCCOC(C(=C)C)=O
|
| HS Tariff Code |
2934.99.9001
|
| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment, avoid exposure to moisture. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
Ethanol: 50 mg/mL
|
|---|---|
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
Products are for research use only; We do not sell to patients
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