| Size | Price | Stock | Qty |
|---|---|---|---|
| 500mg |
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| Other Sizes |
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| ln Vitro |
Ferrous bisglycinate (25–200 μM; 2 h) has no effect on the viability of Caco-2 cells[2]. The Caco-2 cells' ferritin level is increased by ferrous bisglycinate (25 μM; 2 h)[2].
|
|---|---|
| ln Vivo |
Mice treated with ferrous bisglycinate (500 mg/kg iron; po) do not develop colitis[3].
|
| Cell Assay |
Cell Viability Assay[2]
Cell Types: Caco-2 cells Tested Concentrations: 0, 25, 50, 100, 200 μM Incubation Duration: 2 hrs (hours) Experimental Results: Did not affect the viability of wild-type and divalent metal transporter 1 (DMT1) knockout Caco-2 cells . Western Blot Analysis[2] Cell Types: Caco-2 cells Tested Concentrations: 25 μM Incubation Duration: 2 hrs (hours) Experimental Results: Increased ferritin content and diminished DMT1 expression levels Dramatically in the wild-type cells. |
| Animal Protocol |
Animal/Disease Models: Female C57BL/6 mice are induced colitis by dextran sodium sulfate (DSS)[3]
Doses: 500 mg/kg iron Route of Administration: Po (add to the diet) for 10 days Experimental Results: Had the best survival rates (100%). Caused the least body lost (9% body loss). |
| References |
[1]. Ferrari P, et, al. Treatment of mild non-chemotherapy-induced iron deficiency anemia in cancer patients: comparison between oral ferrous bisglycinate chelate and ferrous sulfate. Biomed Pharmacother. 2012 Sep; 66(6): 414-8.
[2]. Yu X, et, al. Iron Transport from Ferrous Bisglycinate and Ferrous Sulfate in DMT1-Knockout Human Intestinal Caco-2 Cells. Nutrients. 2019 Feb 26; 11(3): 485. [3]. Constante M, et, al. Iron Supplements Modulate Colon Microbiota Composition and Potentiate the Protective Effects of Probiotics in Dextran Sodium Sulfate-induced Colitis. Inflamm Bowel Dis. 2017 May; 23(5): 753-766. |
| Molecular Formula |
C4H6FEN2O4
|
|---|---|
| Molecular Weight |
201.95
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| CAS # |
20150-34-9
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| Appearance |
Typically exists as solids (or liquids in special cases) at room temperature
|
| SMILES |
[Fe+2].[O-]C(C([H])([H])N([H])[H])=O.[O-]C(C([H])([H])N([H])[H])=O
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: (1). This product requires protection from light (avoid light exposure) during transportation and storage. (2). Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
0.1 M HCL: 2 mg/mL (9.90 mM)
|
|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: 2 mg/mL (9.90 mM) in PBS (add these co-solvents sequentially from left to right, and one by one), clear solution; with sonication (<60°C).
(Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 4.9517 mL | 24.7586 mL | 49.5172 mL | |
| 5 mM | 0.9903 mL | 4.9517 mL | 9.9034 mL | |
| 10 mM | 0.4952 mL | 2.4759 mL | 4.9517 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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