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1mg |
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5mg |
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10mg |
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Other Sizes |
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ln Vitro |
In HT 29 and HCT 116 cells, ilexgenin A (0~100 μM; 24 hours) significantly reduces cell viability[1]. In HT 29 cells, ilexgenin A (12.5~50 μM; 24 hours) suppresses SREBP-1 expression, prevents SREBP-1 from moving to the nucleus, and lowers TG content[1]. In colon cancer cells, ilexgenin A (12.5~50 μM; HT 29 and HCT 116 cells) causes cell cycle arrest at the G1 phase[1]. Lipid metabolism in HCT 116 cells is modulated by ilexgenin A (12.5~50 μM; 24 hours). Through its inhibition of HIF1α, ilexgenin A (50 μM; 24 hours; HT 29 and HCT 116 cells) regulates SREBP-1[1]. Synthesis of fatty acids is inhibited by ilexgenin A[1].
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ln Vivo |
Ilexgenin A (20 mg/kg) reduces the carcinogenesis caused by AOM and DSS. Lipid metabolism can be regulated by ilexgenin A. In AOM/DSS mice, ilexgenin A reduces the expression of SREBP-1 and HIF 1α[1].
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Cell Assay |
Cell Viability Assay[1]
Cell Types: HT 29 and HCT 116 cells Tested Concentrations: 0~100 μM Incubation Duration: 24 hrs (hours) Experimental Results: Effectively diminished cell viability in HT 29 and HCT 116 cells. Western Blot Analysis[1] Cell Types: HT 29 cells Tested Concentrations: 12.5~50 μM Incubation Duration: 24 hrs (hours) Experimental Results: Downregulated the expression of SREBP-1. RT-PCR[1] Cell Types: HT 29 cells Tested Concentrations: 12.5~50 μM Incubation Duration: 24 hrs (hours) Experimental Results: diminished the content of TG. Immunofluorescence[1] Cell Types: HT 29 cells Tested Concentrations: 12.5~50 μM Incubation Duration: 24 hrs (hours) Experimental Results: Inhibited the translocation of SREBP-1 to the nucleus. |
References |
Molecular Formula |
C30H46O6
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Molecular Weight |
502.68
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Exact Mass |
502.32943918
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CAS # |
108524-94-3
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Appearance |
White to off-white solid
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SMILES |
C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H]([C@]5(C)C(=O)O)O)C)C)[C@@H]2[C@]1(C)O)C)C(=O)O
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InChi Key |
UIEGOKVPCRANSU-XUFMHOFVSA-N
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InChi Code |
InChI=1S/C30H46O6/c1-17-9-14-30(24(34)35)16-15-26(3)18(22(30)29(17,6)36)7-8-19-25(2)12-11-21(31)28(5,23(32)33)20(25)10-13-27(19,26)4/h7,17,19-22,31,36H,8-16H2,1-6H3,(H,32,33)(H,34,35)/t17-,19-,20-,21+,22-,25-,26-,27-,28-,29-,30+/m1/s1
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Chemical Name |
(3S,4R,4aR,6aR,6bS,8aS,11R,12R,12aS,14aR,14bR)-3,12-dihydroxy-4,6a,6b,11,12,14b-hexamethyl-1,2,3,4a,5,6,7,8,9,10,11,12a,14,14a-tetradecahydropicene-4,8a-dicarboxylic acid
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Synonyms |
Ilexgenin A
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HS Tariff Code |
2934.99.9001
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Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage. |
Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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Solubility (In Vitro) |
DMSO: 100 mg/mL (198.93 mM)
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Solubility (In Vivo) |
Solubility in Formulation 1: 2.5 mg/mL (4.97 mM) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), suspension solution; with sonication.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (4.97 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly.  (Please use freshly prepared in vivo formulations for optimal results.) |
Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
1 mM | 1.9893 mL | 9.9467 mL | 19.8934 mL | |
5 mM | 0.3979 mL | 1.9893 mL | 3.9787 mL | |
10 mM | 0.1989 mL | 0.9947 mL | 1.9893 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration: mg/mL;
Method for preparing DMSO stock solution: mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation::Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O,mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.