| Size | Price | Stock | Qty |
|---|---|---|---|
| 5mg |
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| 10mg |
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| 50mg |
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| Other Sizes |
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| Targets |
glycoprotein labeling reagent
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|---|---|
| ln Vitro |
For cell labeling, tracking, and proteome analysis, Ac4ManNAz (10 μM) has enough labeling efficiency with little effect on biological systems [1]. Major cellular processes such as energy production, cell infiltration, and channel activity are all reduced by Ac4ManNAz (50 μM) [1].
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| ln Vivo |
It is suggested that 10 μM should be used as the optimal concentration of Ac4ManNAz for in vivo cell labeling and tracking of hUCB-EPCs. Additionally, we expect that our approach can be used for understanding the efficacy and safety of stem cell-based therapy in vivo and to help determine the utility of stem cells in downstream experiments.[2]
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| Enzyme Assay |
Mitochondrial membrane potential was measured using JC-1 dye (5′,6,6′-tetrachloro-1,1′,3,3′-tetraethylbenzimidazolylcarbocyanine iodide; Life Technologies) according to the manufacturer's instructions. Briefly, Ac4MAnNAz-treated or untreated cells were incubated with 10 µg/mL JC-1 dye for 15 min, and fluorescence images were taken using a 20x objective. The ratio of red fluorescence JC-1 aggregates and green JC-1 monomers was measured using image J following image background correction.[1]
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| Cell Assay |
Invasion and wound healing
Matrigel (100 μL; 7-8 mg/mL) in serum-free medium was added to each well of a Transwell Corning Costar plate (Costar, Acton, MA, USA) and dried overnight in a culture hood. The following day, 2.5 × 104 cells in serum-free medium were pipetted onto the Matrigel, and complete medium was added to the bottom chamber. Following incubation, the transmembrane filter was stained with crystal violet and the number of cells counted. For wound healing, a small area was cleared along the diameter of the 10 cm dishes through confluent monolayers of A549 and Az4MAnNAz-treated A549 cells using a sterile pipette tip. Cell migration was measured and photographed from the wound/scratch edge every 8 h.[1] |
| References |
[1]. Han SS, et, al. Physiological Effects of Ac4ManNAz and Optimization of Metabolic Labeling for Cell Tracking. Theranostics. 2017 Mar 1;7(5):1164-1176.
[2]. Han SS, et, al. Safety and Optimization of Metabolic Labeling of Endothelial Progenitor Cells for Tracking. Sci Rep. 2018 Sep 4;8(1):13212. [3]. Spiciarich DR, et, al. Bioorthogonal Labeling of Human Prostate Cancer Tissue Slice Cultures for Glycoproteomics. Angew Chem Int Ed Engl. 2017 Jul 24;56(31):8992-8997. |
| Molecular Formula |
C16H22N4O10
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|---|---|
| Molecular Weight |
430.37
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| Exact Mass |
430.1336
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| Elemental Analysis |
C, 44.65; H, 5.15; N, 13.02; O, 37.17
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| CAS # |
1213701-11-1
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| Related CAS # |
Ac4ManNAz;361154-30-5
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| Appearance |
Solid powder
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| LogP |
1.2
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| tPSA |
158Ų
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| SMILES |
O([C@H]1[C@@H]([C@@H](COC(=O)C)O[C@H](OC(=O)C)[C@H]1NC(=O)CN=[N+]=[N-])OC(=O)C)C(=O)C
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| InChi Key |
HGMISDAXLUIXKM-LIADDWGISA-N
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| InChi Code |
InChI=1S/C16H22N4O10/c1-7(21)26-6-11-14(27-8(2)22)15(28-9(3)23)13(16(30-11)29-10(4)24)19-12(25)5-18-20-17/h11,13-16H,5-6H2,1-4H3,(H,19,25)/t11-,13+,14-,15-,16?/m1/s1
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| Chemical Name |
Peracetylated N-azidoacetyl-d-mannosamine
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| HS Tariff Code |
2934.99.03.00
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO: 100 mg/mL (232.36 mM)
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|---|---|
| Solubility (In Vivo) |
≥ 2.50 mg/mL (5.80 mM) in 10% DMSO + 40% PEG300 + 5% Tween-80 + 45% Saline
≥ 2.50 mg/mL (5.80 mM) in 10% DMSO + 90% (20% SBE-β-CD in saline) ≥ 2.50 mg/mL (5.80 mM) in 10% DMSO + 90% Corn oil (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.3236 mL | 11.6179 mL | 23.2358 mL | |
| 5 mM | 0.4647 mL | 2.3236 mL | 4.6472 mL | |
| 10 mM | 0.2324 mL | 1.1618 mL | 2.3236 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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