Size | Price | Stock | Qty |
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5mg |
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10mg |
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50mg |
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Other Sizes |
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ln Vitro |
In HEK293 cells expressing GFP-HaloTag2/Fz4-HaloTag2/GFP-HaloTag7 fusion protein, HyT36 (10 µM; 24 h) decreases the expression of GFP-HaloTag2/Fz4-HaloTag2 [1]. In HEK293 Flp-In cells expressing HA-EGFP-HaloTag2, HyT36 (10 µM; 2 h or 48 h) decreased EGFP expression and increased Hsp70 binding to the HA-EGFP-HaloTag2 fusion protein [3]. In HEK293 Flp-In cells expressing HA-EGFP-HaloTag2, HyT36 (50 nM; 24 h) lowers the amount of EGFP-HaloTag2 [3]. In NIH-3T3 cells expressing HA−HaloTag2−HRasG12V, HyT36 (1 µM; 4 d) decreases the expression of HA−HaloTag2−HRasG12V [
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Cell Assay |
Western Blot Analysis[1][3]
Cell Types: HEK293 cells expressing GFP-HaloTag2/Fz4-HaloTag2/HA-EGFP-HaloTag2 or NIH-3T3 cells expressing HA−HaloTag2−HRasG12V. Tested Concentrations: 1 µM or 10 µM. Incubation Duration: 2 h, 24 h or 4 days. Experimental Results: diminished the expression of fusion proteins and increased the binding of Hsp70 and HA-EGFP-HaloTag2 fusion protein. |
References |
[1]. Tae HS, et al. Identification of hydrophobic tags for the degradation of stabilized proteins. Chembiochem. 2012 Mar 5;13(4):538-41.
[2]. Rubner S, et al. Poloxin-2HT+: changing the hydrophobic tag of Poloxin-2HT increases Plk1 degradation and apoptosis induction in tumor cells. Org Biomol Chem. 2019 Mar 20;17(12):3113-3117. [3]. Neklesa TK, et al. A bidirectional system for the dynamic small molecule control of intracellular fusion proteins. ACS Chem Biol. 2013 Oct 18;8(10):2293-2300. |
Molecular Formula |
C25H44CLNO3
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Molecular Weight |
442.07
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CAS # |
1323151-45-6
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Appearance |
Typically exists as solids (or liquids in special cases) at room temperature
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SMILES |
ClC([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])OC([H])([H])C([H])([H])OC([H])([H])C([H])([H])N([H])C(C([H])(C([H])([H])[H])C([H])([H])C([H])([H])C12C([H])([H])C3([H])C([H])([H])C([H])(C([H])([H])C([H])(C3([H])[H])C1([H])[H])C2([H])[H])=O
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HS Tariff Code |
2934.99.9001
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Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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Solubility (In Vitro) |
DMSO: 100 mg/mL (226.21 mM)
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Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (5.66 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (5.66 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C,1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. View More
Solubility in Formulation 3: ≥ 2.5 mg/mL (5.66 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. |
Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
1 mM | 2.2621 mL | 11.3104 mL | 22.6209 mL | |
5 mM | 0.4524 mL | 2.2621 mL | 4.5242 mL | |
10 mM | 0.2262 mL | 1.1310 mL | 2.2621 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration: mg/mL;
Method for preparing DMSO stock solution: mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation::Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O,mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.