| Size | Price | Stock | Qty |
|---|---|---|---|
| 100mg |
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| 500mg |
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| Other Sizes |
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| ln Vitro |
Method of use: 1.1 Prepare the stock solution by mixing 10 mM DPBF with DMSO. 2. Prepare the 1,3-Diphenylisobenzofuran (DPBF) working solution. For instance, 10 mg of DPBF can be dissolved in 3.7 mL of DMSO. Note: It is advised to aliquot the DPBF storage solution and keep it at -20°C or -80°C in the dark. 1.2 Making the working solution Prepare a 10–20 μM DPBF working solution using either PBS diluted storage solution or hot serum-free cell culture medium. Note: Please prepare the DPBF working fluid for usage by adjusting its concentration to suit the current circumstances. 2.1 Suspension cells for cell staining: Centrifuge cells, add PBS, and wash twice for five minutes each time. Trypsin should be added in order to break down adherent cells after discarding the culture media. Centrifuge, remove supernatant, add PBS, and wash twice for five minutes each time. Note: Adherent cells do not require digestion if flow cytometry experiments are conducted. 2.2 Add 1 milliliter of DPBF working solution, then let it sit at room temperature for half an hour. 2.3 Discard the supernatant after centrifuging at 400 g for three to four minutes at 4°C. 2.4 After adding PBS, wash the cells twice for five minutes each. 2.5 Re-suspend the cells in 1 milliliter of PBS or serum-free media, and examine using a flow cytometer or fluorescence microscope. Observations 1. To prevent recurrent freezing and thawing, it is advised to aliquot the DPBF storage solution and store it in the dark at -20°C or -80°C. 2. Kindly modify the DPBF working fluid concentration based on the current circumstances. 3. This product may not be used for clinical diagnosis or treatment, nor may it be included into food or medication. It is intended solely for professional use in scientific study. 4. Please wear disposable gloves and a lab coat for your health and safety.
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| References |
[1]. Bai X, et al. HKOH-1: A Highly Sensitive and Selective Fluorescent Probe for Detecting Endogenous Hydroxyl Radicals in Living Cells. Angew Chem Int Ed Engl. 2017 Oct 9;56(42):12873-12877.
[2]. Weili Fan, et al. Calcium carbonate-methylene blue nanohybrids for photodynamic therapy and ultrasound imaging. Sci China Life Sci. 2018 Apr;61(4):483-491. [3]. P. Carloni, et al. On the use of 1,3-diphenylisobenzofuran (DPBF). Reactions with carbon and oxygen centered radicals in model and natural systems. Research on Chemical Intermediates volume 19, pages395-405(1993). |
| Molecular Formula |
C20H14O
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|---|---|
| Molecular Weight |
270.33
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| CAS # |
5471-63-6
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| SMILES |
O1C(C2C([H])=C([H])C([H])=C([H])C=2[H])=C2C([H])=C([H])C([H])=C([H])C2=C1C1C([H])=C([H])C([H])=C([H])C=1[H]
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| Solubility (In Vitro) |
DMSO: 5.56 mg/mL (20.57 mM)
H2O: < 0.1 mg/mL |
|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: 0.56 mg/mL (2.07 mM) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), suspension solution; with sonication.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 5.6 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. Solubility in Formulation 2: 0.56 mg/mL (2.07 mM) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), suspension solution; with ultrasonication. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 5.6 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 3.6992 mL | 18.4959 mL | 36.9918 mL | |
| 5 mM | 0.7398 mL | 3.6992 mL | 7.3984 mL | |
| 10 mM | 0.3699 mL | 1.8496 mL | 3.6992 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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