Size | Price | Stock | Qty |
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1mg |
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5mg |
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10mg |
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Other Sizes |
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ln Vitro |
Advice (This is our suggested protocol, which should be adjusted to suit your particular circumstances as it simply offers guidance). SELP analogues conjugated to Cy5-SE. 1. Combine 10 μL of 1.2 mg/mL Cy5 monoNHS-ester in DMSO with 1 mL of 100 μg/mL SELP solution in 100 mM sodium bicarbonate buffer (pH 8.3), then let it sit on ice for two hours. ..2. To quench the reaction, add 50 μL of 1 M Tris-HCl (pH 8.0) to the reaction solution. 3. Centrifugation at 1050g[1] for three minutes is the method used to elute the Cy5-conjugated SELP after loading the reaction mixture onto a 1.5 mL Sephadex G-25 column.
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References |
[1]. Jeon HY, et al. Array-Based High-Throughput Analysis of Silk-Elastinlike Protein Polymer Degradation and C-Peptide Release by Proteases. Anal Chem. 2016;88(10):5398-5405.
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Molecular Formula |
C43H58N4O10S2
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Molecular Weight |
855.07
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CAS # |
1497420-70-8
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Related CAS # |
CY5-SE;146368-14-1
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Appearance |
Typically exists as solids (or liquids in special cases) at room temperature
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SMILES |
S(C1C=CC2=C(C=1)C(C)(C)C(/C=C/C=C/C=C1\C(C)(C)C3C=C(C=CC=3N\1CC)S(=O)(=O)[O-])=[N+]2CCCCCC(=O)ON1C(CCC1=O)=O)(=O)(=O)O.N(CC)(CC)CC
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HS Tariff Code |
2934.99.9001
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Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture and light. |
Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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Solubility (In Vitro) |
DMSO: 125 mg/mL (146.19 mM)
H2O: ≥ 5.88 mg/mL (6.88 mM) |
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Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.08 mg/mL (2.43 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C,1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. Solubility in Formulation 2: 25 mg/mL (29.24 mM) in Saline (add these co-solvents sequentially from left to right, and one by one), clear solution; with ultrasonication (<60°C). Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution.  (Please use freshly prepared in vivo formulations for optimal results.) |
Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
1 mM | 1.1695 mL | 5.8475 mL | 11.6949 mL | |
5 mM | 0.2339 mL | 1.1695 mL | 2.3390 mL | |
10 mM | 0.1169 mL | 0.5847 mL | 1.1695 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration: mg/mL;
Method for preparing DMSO stock solution: mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation::Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O,mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.