| Size | Price | Stock | Qty |
|---|---|---|---|
| 1mg |
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| 5mg |
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| Other Sizes |
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| ln Vitro |
1. 1.1 Creating the working solution for HKPerox-1 Dilute 1 milligram of HKPerox-1 with 140 μL of anhydrous DMF to create a 10 mM stock solution. It is advised that HKPerox-1 storage solution be aliquoted and kept in the dark at -20°C or -80°C. 1.2 Making the working solution: To make a 1-10 μM HKPerox-1 working solution, use warmed serum-free cell culture medium or PBS dilution stock solution. Note: Please be ready for instant use by adjusting the HKPerox-1 working fluid concentration based on the current circumstances. 2. Staining cells (suspension cells) 2.1 Centrifuge the cells, add PBS, then wash twice for five minutes each. One x 10^6 cells per milliliter. 2.2 After adding 1 mL of HKPerox-1 working solution, let it sit at room temperature for a duration of 5 to 30 minutes. 2.3 Discard the supernatant after centrifuging for 3–4 minutes at 400 g. 2.4 Add PBS and give the cells two 5-minute washing cycles. 2.5 Use a flow cytometer or fluorescence microscope to view the cells after resuspending them in 1 mL of serum-free media or PBS. 3. 3. Culture adherent cells on sterile coverslips for cell labeling (adherent cells). 3.2 Take off the coverslip from the culture media and use an aspirator to remove any extra. 3.3 Add 100 μL of the dye working solution, give the cells a gentle shake to cover them completely, and then let them sit for five to thirty minutes. 3.4 Aspirate the dye working solution, rinse with culture medium two or three times for five minutes each time, and use a flow cytometer or fluorescence microscope to monitor.
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| References |
| Molecular Formula |
C40H30N2O11
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|---|---|
| Molecular Weight |
714.673011302948
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| Exact Mass |
714.184
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| CAS # |
2240163-05-5
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| PubChem CID |
162640794
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| Appearance |
Yellow to orange solid powder
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| LogP |
6
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| Hydrogen Bond Donor Count |
2
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| Hydrogen Bond Acceptor Count |
11
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| Rotatable Bond Count |
10
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| Heavy Atom Count |
53
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| Complexity |
1280
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| Defined Atom Stereocenter Count |
0
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| SMILES |
O=C1C2=CC=CC=C2C2(C3=CC=C(N(C)C(=O)OCC4C=CC(C=O)=C(O)C=4)C=C3OC3=CC(N(C)C(=O)OCC4C=CC(C=O)=C(O)C=4)=CC=C23)O1
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| InChi Key |
WOSPPMBMPHNEFP-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C40H30N2O11/c1-41(38(48)50-21-23-7-9-25(19-43)33(45)15-23)27-11-13-31-35(17-27)52-36-18-28(42(2)39(49)51-22-24-8-10-26(20-44)34(46)16-24)12-14-32(36)40(31)30-6-4-3-5-29(30)37(47)53-40/h3-20,45-46H,21-22H2,1-2H3
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| Chemical Name |
(4-formyl-3-hydroxyphenyl)methyl N-[6'-[(4-formyl-3-hydroxyphenyl)methoxycarbonyl-methylamino]-3-oxospiro[2-benzofuran-1,9'-xanthene]-3'-yl]-N-methylcarbamate
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMF: 50 mg/mL (69.96 mM)
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|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: 2.5 mg/mL (3.50 mM) = in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), suspension solution; with sonication.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (3.50 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly.= View More
Solubility in Formulation 3: 1.25 mg/mL (1.75 mM) in 10% DMF 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), suspension solution; with ultrasonication. Solubility in Formulation 4: ≥ 1.25 mg/mL (1.75 mM) (saturation unknown) in 10% DMF 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 1.3992 mL | 6.9962 mL | 13.9925 mL | |
| 5 mM | 0.2798 mL | 1.3992 mL | 2.7985 mL | |
| 10 mM | 0.1399 mL | 0.6996 mL | 1.3992 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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